Antibiofilm and anti-caries effects of an experimental mouth rinse containing Matricaria chamomilla L. extract under microcosm biofilm on enamel

Autor(es)

Braga, Aline Silva ^[1] ; Simas, Leticia Lobo de Melo ^[1] ; Pires, Juliana Gonsalves ^[1] ; Souza, Beatriz Martines ^[1] ; Melo, Fernanda Pereira de Souza Rosa de ^[2] ; Saldanha, Luiz Leonardo ^[2] ; Dokkedal, Anne Ligia ^[2] ; Magalhaes, Ana Carolina ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Al Octavio Pinheiro Brisolla 9-75, BR-17012901 Bauru, SP - Brazil

^[2] Sao Paulo State Univ UNESP, Sch Sci, Dept Biol Sci, Bauru, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Dentistry; v. 99, AUG 2020.
• Citações Web of Science: 0

Resumo

Objective: This study evaluated the antibiofilm and anti-caries effects of an experimental mouth rinse containing aqueous extract of Matricaria chamomilla L. Methods: Microcosm biofilm was produced on bovine enamel, from pooled human saliva mixed with McBain saliva, under 0.2 % sucrose exposure, for 5 days. The biofilm was daily treated using (1 mL/1 min): Vochysia tucanorum Mart. (2.5 mg/mL); Myrcia bella Cambess. (1.25 mg/mL); Matricaria chamomilla L. (20 mg/mL); Malva sylvestris (Malvatricin (R) Plus-Daudt); 0.12 % Chlorhexidine (PerioGard (R)-Palmolive, Positive control) and PBS (Negative control). The % dead bacteria, biofilm thickness, EPS biovolume, lactic acid concentration, the CFU counting (total microorganisms, Lactobacillus sp., total streptococci and Streptococcus mutans/S. sobrinus) were determined. Enamel demineralization was measured by TMR. Results: All mouth rinses induced bacterial death compared to PBS (p < 0.0001). The biofilm thickness varied from 12 +/- 2 mu m (chlorhexidine) to 18 +/- 2 mu m (V. tucanorum) (ANOVA/Tukey, p < 0.0001). The EPS biovolume varied from 7(4)% (chlorhexidine) to 30(20)% (PBS) (Kruskal-Wallis/Dunn, p < 0.0001). The lactic acid production was reduced by M. sylvestris (1.1 +/- 0.2 g/L) and chlorhexidine (0.6 +/- 0.2 g/L) compared to PBS (2.6 +/- 1.3 g/L) (ANOVA, p < 0.0001). Malva sylvestris and chlorhexidine showed significant low CFU for total microorganisms, Lactobacillus sp. and total streptococci. Only chlorhexidine significantly reduced S. mutans/S. sobrinus. CFUs for total streptococci and Lactobacillus sp, were also significantly reduced by M. chamomilla L. Malva sylvestris (63.4 % of mineral loss reduction), chlorhexidine (47.4 %) and M. chamomilla L. (39.4 %) significantly reduced enamel demineralization compared to PBS (ANOVA/Tukey, p < 0.0001). Conclusion: M. chamomilla L. has lower antibiofilm action, but comparable anti-caries effect to those found for chlorhexidine, under this model. (AU)

• Processo FAPESP: 17/00556-0 - Avaliação do efeito antimicrobiano de agentes comerciais e alternativos sobre biofilme microcosmo e sobre a prevenção da desmineralização dentária
• Beneficiário: Ana Carolina Magalhães
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/17249-2 - Avaliação do efeito de diferentes extratos naturais experimentais e comerciais, na forma de dentifrícios e enxaguatórios bucais, sobre a viabilidade e atividade de biofilme microcosmo e sobre a prevenção da desmineralização do esmalte in vitro
• Beneficiário: Aline Silva Braga
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/23165-6 - Avaliação do efeito de diferentes extratos naturais, na forma de enxaguatórios bucais experimentais e comerciais, sobre a viabilidade de biofilme microcosmo e prevenção da desmineralização do esmalte
• Beneficiário: Leticia Lobo de Melo Simas
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica