Effects of multiwalled carbon nanotubes co-exposure with cadmium on zebrafish cell line: Metal uptake and accumulation, oxidative stress, genotoxicity and cell cycle

Autor(es)

Morozesk, Mariana ^{[1, 2]} ; Franqui, Lidiane S. ^{[3, 4]} ; Pinheiro, Fernanda C. ^[5] ; Nobrega, Joaquim A. ^[5] ; Martinez, Diego S. T. ^{[3, 4]} ; Fernandes, Marisa N. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Fed Univ Sao Carlos UFSCar, Postgrad Program Ecol & Nat Resources, Av Washington Luiz Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[2] Fed Univ Sao Carlos UFSCar, Physiol Sci Dept, Washington Luiz Av, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[3] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Nanotechnol Natl Lab, Giuseppe Maximo Scolfaro St 10-000, BR-13083970 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Sch Technol, UNICAMP, Paschoal Marmo St 1888, BR-13484332 Limeira, SP - Brazil

^[5] Fed Univ Sao Carlos UFSCar, Chem Dept, Washington Luiz Av, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY; v. 202, OCT 1 2020.
• Citações Web of Science: 0

Resumo

Carbon nanotubes presence in the environment increases every year because of exponential industrial production around the world. In aquatic environments, carbon nanotubes can interact with other pollutants based on their adsorbent surface chemistry properties. Heavy metal ions represent one of the biggest concerns in water resources nowadays due to anthropogenic activities, in which cadmium (Cd) is one of the most harmful metal for aquatic organisms. This study investigated the influence of two co-exposure protocols differing by the order of interaction of oxidized multiwalled carbon nanotubes (ox-MWCNT) with Cd in zebrafish liver cell line (ZFL). The ox-MWCNT was characterized, Cd content in culture medium and uptake by cells were quantified using ICP-MS and, the reactive oxygen species (ROS), the biotransformation enzymes activity of phase I and II as well as the antioxidants defenses and oxidative damage were analyzed. The effects on the cell cycle were investigated by flow cytometry and DNA damage by comet assay. The exposure to ox-MWCNT alone decreased the activity of catalase, glutathione peroxidase, and glutathione S-transferase and altered the cell cycle with a reduction of cells in the G2/M phase. Cd exposure alone decreased the activity of catalase and glutathione S-transferase, increased ROS, metallothionein, and lipid peroxidation content and causes genotoxicity in the cells. Despite different incubation protocol, the co-exposure ox-MWCNT-Cd increased the Cd content in ZFL cells after 24 h exposure, increased ROS production and DNA damage without differences between them. Our results showed the modulation of ox-MWCNT on Cd effects and contributed to future co-exposure toxicity investigations and nanosafety regulations involving carbon nanomaterials and aquatic pollutants. (AU)

• Processo FAPESP: 14/50906-9 - INCT 2014: em Materiais Complexos Funcionais (INOMAT)
• Beneficiário: Fernando Galembeck
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/05701-0 - Respostas integradas de curimbatá, Prochilodus lineatus (Teleostei) às nanopartículas de dióxido de titânio (TiO2) e identificação de biomarcadores para o monitoramento ambiental
• Beneficiário: Marisa Narciso Fernandes
• Modalidade de apoio: Auxílio à Pesquisa - Temático