Costus stripe mosaic virus, a tentative new member of the genus Potyvirus

Autor(es)

Alexandre, M. Amelia V. ^[1] ; Duarte, Ligia M. L. ^[1] ; Ramos-Gonzalez, Pedro L. ^[2] ; Chaves, Alexandre L. R. ^[1] ; Harakava, Ricardo ^[2] ; Rodrigues, Leilane K. ^[1] ; Chabi-Jesus, Camila ^{[3, 2]} ; Kitajima, E. W. ^[3]

Afiliação do(s) autor(es)

^[1] Inst Biol, Lab Fitovirol Fisiopatol, Ave Cons Rodrigues Alves 1252, BR-04014002 Sao Paulo, SP - Brazil

^[2] Inst Biol, Lab Biol Mol Aplicada, Ave Cons Rodrigues Alves 1252, BR-04014002 Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, CP 09, BR-13418900 Piracicaba, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ARCHIVES OF VIROLOGY; v. 165, n. 11 AUG 2020.
• Citações Web of Science: 0

Resumo

Morphological, biological, serological, and molecular tests underpin the description of costus stripe mosaic virus (CoSMV) as a new member of the genus Potyvirus, family Potyviridae. Found affecting the native ornamental Costus spiralisin Brazil, the pathogen showed a severely restricted natural and experimental host range. Excluding the poly(A) tail, the CoSMV genome contains a large open reading frame (ORF) of 9,446 nucleotides that encodes a polyprotein with 3,046 amino acids, which is potentially cleaved into ten products, and a small ORF (77 amino acids) knows as PIPO. Genome analysis demonstrated the highest CoSMV nucleotide sequence identity to onion yellow dwarf virus (51.79%). No evidence of recombination was detected in the CoSMV genome, and phylogenetic analysis revealed its basal position in a group formed by members of the genusPotyvirus, along with Cyrtanthus elatus virus A (Vallota speciosa virus) and canna yellow streak virus. CoSMV was not transmitted by aphids of the species Aphis solanella, Myzus persicaeor Uroleucon sonchi, which could be due to mutations in the HC-Pro motifs required for aphid transmission. A divergence in the P1 protein cleavage site was found when compared to other members of the family Potyviridae. Based on its unique biological and molecular characteristics and the current species demarcation criteria, we propose CoSMV to be a new tentative member of the genus Potyvirus. (AU)

• Processo FAPESP: 17/18910-4 - Avaliação da diversidade dos patógenos moleculares (vírus e viróides de plantas) no Brasil: fase II-2018
• Beneficiário: Elliot Watanabe Kitajima
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Regular