In-depth characterization of a novel live-attenuated Mayaro virus vaccine candidate using an immunocompetent mouse model of Mayaro disease

Autor(es):Mostrar menos -

de Oliveira Mota, Manlio Tasso ^[1] ; Costa, Vivian Vasconcelos ^[2] ; Sugimoto, Michelle Amantea ^[2] ; Guimaraes, Georgia de Freitas ^[1] ; Queiroz-Junior, Celso Martins ^[2] ; Moreira, Thaiane Pinto ^[2] ; de Sousa, Carla Daiane ^[2] ; Santos, Franciele Martins ^[2] ; Queiroz, Victoria Fulgencio ^[2] ; Passos, Ingredy ^[2] ; Hubner, Josy ^[2] ; Souza, Danielle Gloria ^[2] ; Weaver, Scott C. ^{[3, 4]} ; Teixeira, Mauro Martins ^[2] ; Nogueira, Mauricio Lacerda ^[1]

Afiliação do(s) autor(es)

^[1] Fac Med Sao Jose do Rio Preto, Sao Jose Do Rio Preto, SP - Brazil

^[2] Univ Fed Minas Gerais, Inst Biol Sci, Belo Horizonte, MG - Brazil

^[3] Univ Texas Med Branch, Dept Microbiol & Immunol, Galveston, TX 77555 - USA

^[4] Univ Texas Med Branch, Inst Human Infect & Immun, World Reference Ctr Emerging Viruses & Arboviruse, Galveston, TX 77555 - USA

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 10, n. 1 MAR 24 2020.
• Citações Web of Science: 5

Resumo

Mayaro virus (MAYV) is endemic in South American countries where it is responsible for sporadic outbreaks of acute febrile illness. The hallmark of MAYV infection is a highly debilitating and chronic arthralgia. Although MAYV emergence is a potential threat, there are no specific therapies or licensed vaccine. In this study, we developed a murine model of MAYV infection that emulates many of the most relevant clinical features of the infection in humans and tested a live-attenuated MAYV vaccine candidate (MAYV/IRES). Intraplantar inoculation of a WT strain of MAYV into immunocompetent mice induced persistent hypernociception, transient viral replication in target organs, systemic production of inflammatory cytokines, chemokines and specific humoral IgM and IgG responses. Inoculation of MAYV/IRES in BALB/c mice induced strong specific cellular and humoral responses. Moreover, MAYV/ IRES vaccination of immunocompetent and interferon receptor-defective mice resulted in protection from disease induced by the virulent wt MAYV strain. Thus, this study describes a novel model of MAYV infection in immunocompetent mice and highlights the potential role of a live-attenuated MAYV vaccine candidate in host's protection from disease induced by a virulent MAYV strain. (AU)

• Processo FAPESP: 14/05600-9 - Caracterização inicial da resposta imune elicitada por candidato a vacina para febre Mayaro
• Beneficiário: Mânlio Tasso de Oliveira Mota
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/21719-3 - Estudo epidemiológico da dengue (sorotipos 1 a 4) em coorte prospectiva de São José do Rio Preto, São Paulo, Brasil, durante 2014 a 2018
• Beneficiário: Maurício Lacerda Nogueira
• Modalidade de apoio: Auxílio à Pesquisa - Temático