Predicting metabolic pathways of plant enzymes without using sequence similarity: Models from machine learning

Autor(es)

Almeida, Rodrigo de Oliveira ^{[1, 2]} ; Valente, Guilherme Targino ^{[2, 3]}

Afiliação do(s) autor(es)

^[1] Inst Fed Educ Ciencia & Tecnol Sudeste Minas Gera, Muriae - Brazil

^[2] Sao Paulo State Univ, Sch Agr, Dept Bioproc & Biotechnol, UNESP, Botucatu, SP - Brazil

^[3] Max Planck Inst Heart & Lung Res, Dept Dev Genet, Bad Nauheim - Germany

• Tipo de documento: Artigo Científico
• Fonte: PLANT GENOME; v. 13, n. 3 AUG 2020.
• Citações Web of Science: 0

Resumo

Most of the bioinformatics tools for enzyme annotation focus on enzymatic function assignments. Sequence similarity to well-characterized enzymes is often used for functional annotation and to assign metabolic pathways. However, these approaches are not feasible for all sequences leading to inaccurate annotations or lack of metabolic pathway information. Here we present the mApLe (metabolic pathway predictor of plant enzymes), a high-performance machine learning-based tool with models to label the metabolic pathway of enzymes rather than specifying enzymes' reactions. The mApLe uses molecular descriptors of the enzyme sequences to perform predictions without considering sequence similarities with reference sequences. Hence, mApLe can classify a diversity of enzymes, even the ones without any homolog or with incomplete EC numbers. This tool can be used to improve the quality of genomic annotation of plants or to narrow down the number of candidate genes for metabolic engineering researches. The mApLe tool is available online, and the GUI can be locally installed. (AU)

• Processo FAPESP: 17/08463-0 - Biologia sistêmica e biologia sintética para a re-engenharia de vias metabólicas em Saccharomyces cerevisiae: lidando com a tolerância ao etanol
• Beneficiário: Guilherme Targino Valente
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular
• Processo FAPESP: 15/12093-9 - Análises integrativas aplicadas à tolerância ao etanol em linhagens de Saccharomyces cerevisiae: uma abordagem envolvendo transcriptoma, proteômica, biologia de sistemas e aprendizado de máquina
• Beneficiário: Guilherme Targino Valente
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Regular