Study of supercritical carbon dioxide pretreatment processes on green coconut fiber to enhance enzymatic hydrolysis of cellulose

Autor(es)

Putrino, Fernando Marques ^[1] ; Tedesco, Marcela ^[1] ; Bodini, Renata Barbosa ^[1] ; de Oliveira, Alessandra Lopes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Anim Sci & Food Engn FZEA, Dept Food Engn, Av Duque de Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 309, AUG 2020.
• Citações Web of Science: 0

Resumo

Green coconut fiber was treated by supercritical CO2 with the aim to enhance hydrolysis of its enzymatic cellulose. To this end, different static conditions of CO2 contact times (3 and 5 h) and polarity modifiers (NaOH, NaHSO4, ethanol) were evaluated at 20 MPa, 70 degrees C and 1 h of dynamic extraction followed by fast depressurization. After supercritical CO2 exposition, SEM images showed fiber damage and FTIR spectra showed decreases of phenolic and wax contents, including a reduction in the degree of the hydrogen bond established between lignin and cellulose. Despite the apparent delignification, supercritical CO2 did not enhance cellulose enzymatic hydrolyses. Fiber exposed to supercritical CO2 (5 h) demonstrated that the highest sugar content (540.9 mu mol glucose likely limited supercritical CO2 delignification; however, green coconut in natura can be an innovative substrate for fermentation in alcohol production. (AU)

• Processo FAPESP: 10/16665-3 - Extração de óleo de grãos de café verdes por fluido supercrítico, fluido pressurizado e comparação de sua composição com o óleo de café obtido por prensagem dos grãos pelos pequenos produtores do estado de São Paulo - análise da concentração de diterpenos
• Beneficiário: Alessandra Lopes de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular