| Texto completo | |
| Autor(es): |
N. Nunez, Luis Fabian
[1, 2]
;
Santander-Parra, Silvana H.
[1]
;
de la Torre, David I.
[1, 3]
;
Sa, Lilian R. M. de
[1]
;
Buim, Marcos R.
[4]
;
Astolfi-Ferreira, Claudete S.
[1]
;
Piantino Ferreira, Antonio J.
[1]
Número total de Autores: 7
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Vet Med, Dept Pathol, Av Prof Dr Orlando M Paiva 87, BR-05508270 Sao Paulo, SP - Brazil
[2] Univ Las Amer UDLA, Carrera Med Vet, Fac Ciencias Salud, Av Jose Queri, Quito 170513 - Ecuador
[3] Cent Univ Ecuador, Inst Res Biomed, E-170201 Quito - Ecuador
[4] Inst Biol, Lab Avian Dis, Av Gaspar Ricardo 1700, BR-17690000 Bastos, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PATHOGENS; v. 9, n. 8 AUG 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Chicken parvovirus (ChPV) is an agent frequently associated with runting stunting syndrome (RSS). This syndrome has been reported in association with ChPV in many countries, including Brazil; however, studies characterizing the virus on a molecular level are scarce, and ChPV pathogenicity in day-old chicks remains unclear. The aim of the present work was to establish the molecular characteristics of ChPV, determine the pathogenicity of ChPV in SPF chicks and detect and quantify ChPV by qPCR in several tissues and chicks of different ages. The experimental challenge was performed at one day of age, and daily and weekly observations were performed and five birds from each experimental group (mock and infected birds) were euthanized to perform the different analysis. ChPV genome copies were detected and quantified by qPCR in gut, spleen, thymus, kidney, pancreas, proventriculus and bursa. Clinically, the infected group presented with diarrhea 24 h post-infection, which persisted until 42 days of age. The small intestine was distended, and its contents were aqueous and foamy. Enteritis and dilated crypts with cyst shapes were observed in intestinal segments. Acute pancreatitis associated with lymphocytic nodules, infiltrating lymphocytes and plasma cells between the pancreatic acinus was observed. Koch's postulate was demonstrated and the genetic characterization of the VP1 gene showed that the Brazilian ChPV isolate belongs to the ChPV II group. (AU) | |
| Processo FAPESP: | 15/09348-5 - Detecção molecular, quantificação, distribuição tecidual e determinação das alterações patológicas de vírus entéricos relacionados com doença entérica em galinhas. |
| Beneficiário: | Luis Fabian Nuñez Naranjo |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 13/08560-5 - Estudo da patogenicidade de amostras de vírus entéricos de origem aviária (astrovírus, vírus da nefrite aviária e parvovírus) em aves infectadas experimentalmente |
| Beneficiário: | Antonio José Piantino Ferreira |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |