Parvalbumin and Ubiquitin as Potential Biomarkers of Mercury Contamination of Amazonian Brazilian Fish

Autor(es)

Souza Vieira, Jose Cavalcante ^{[1, 2]} ; de Oliveira, Grasieli ^[1] ; Braga, Camila Pereira ^[3] ; Fernandes, Mileni da Silva ^[4] ; de Moraes, Paula Martin ^[5] ; Rabelo Buzalaf, Marilia Afonso ^[4] ; Silva de Oliveira, Lincoln Carlos ^[2] ; Padilha, Pedro de Magalhaes ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Botucatu, SP - Brazil

^[2] Fed Univ Mato Grosso do Sul UFMS, Campo Grande, MS - Brazil

^[3] Univ Nebraska, Dept Biochem, Lincoln, NE 68583 - USA

^[4] Univ Sao Paulo, USP, Bauru, SP - Brazil

^[5] Dom Bosco Catholic Univ UCDB, Local Dev, Campo Grande, MS - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOLOGICAL TRACE ELEMENT RESEARCH; v. 197, n. 2, p. 667-675, OCT 2020.
• Citações Web of Science: 1

Resumo

Recent studies have demonstrated the association of mercury (Hg) with some fish proteins, milk, and hair from individuals exposed to the element in the Amazon. However, few studies involve identifying biomarkers of mercury exposure. Therefore, the present study aimed to identify potential biomarkers of Hg exposure in fish. For this, the muscular tissues of two species of fish (Prochilodus lineatusandMylossoma duriventre) that feed the Amazonian human population were analyzed. Through the analyses obtained by graphite furnace atomic absorption spectrometry (GFAAS), it was possible to identify four protein SPOTS where mercury was present. These SPOTS, identified by mass spectrometry (ESI-MS/MS), included parvalbumin and ubiquitin-40S ribosomal protein S27a, and these being metalloproteins with biomarker characteristics. In addition, the results show the intense Hg/protein ratio observed in the two proteins, which makes metalloproteins strong candidates for biomarkers of mercury exposure. Graphical (AU)

• Processo FAPESP: 13/21297-1 - Metalotioneínas em peixes da região amazônica: estudo metalômico associado ao mercúrio
• Beneficiário: Pedro de Magalhães Padilha
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/02668-1 - Utilização de estratégias metaloproteômicas para avaliação de metalotioneínas em amostras de tecido hepático de peixes coletados no reservatório do Ahe Jirau - Bacia do Rio Madeira, RO, Brasil
• Beneficiário: José Cavalcante Souza Vieira
• Linha de fomento: Bolsas no Brasil - Doutorado