Osteoporosis Affects Functional Activity and Gene Expression of Osteoblastic Cells Derived from Rat Alveolar Bone

Autor(es)

Paula Katherine Vargas-Sanchez ^[1] ; Roger Rodrigo Fernandes ^[2] ; Flávia Aparecida Chaves Furlaneto ^[3] ; Luiz Gustavo de Sousa ^[4] ; Selma Siéssere ^[5] ; Karina Fittipaldi Bombonato-Prado ^[6]

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral Biology - Brasil

^[2] Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral Biology - Brasil

^[3] Universidade de São Paulo. Department of Oral and Maxillofacial Surgery and Periodontology, School of Dentistry - Brasil

^[4] Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral Biology - Brasil

^[5] Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral Biology - Brasil

^[6] Universidade de São Paulo. School of Dentistry. Bone Research Lab, Department of Basic and Oral Biology - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Dental Journal; v. 31, n. 6, p. 617-622, 2020-11-23.

Resumo

Abstract Recent studies suggest that osteoporosis, in addition to the damage caused in long bones, may cause deterioration in the jaws, especially in alveolar bone sites, with effects in the progress of periodontal disease as well as in bone healing. The aim of this study was to evaluate the effect of osteoporosis in the metabolism of rat alveolar bone osteoblasts. There were used 10 female rats divided in two experimental groups (Sham and OVX), which were ovariectomized and after 8 weeks euthanized to collect mandibular bone samples in order to isolate osteoblastic cells. The cells were cultured in 24-well plates to perform the in vitro experiments. After 7, 10 and 14 days, there were evaluated cell proliferation by MTT assay, in situ detection of alkaline phosphatase (ALP) as well as mineralized nodules and expression of genes associated to bone remodeling. Results showed that at 7, 10 and 14 days cell proliferation was lower for OVX group. In situ detection of ALP was higher at 7 days and lower at 10 and 14 days in OVX group. At 17 and 21 days, OVX group had a significative decrease of mineralization nodules. There was a downregulation in the expression of Alp, Bglap and Runx2 genes and an upregulation of Opg in OVX group, whereas Opn and Rankl modulation was similar between the evaluated groups. Our results suggest that osteoporosis has a deleterious effect on alveolar bone cells from ovariectomized rats, which might affect the treatment of diseases associated to the jaw bones. (AU)

• Processo FAPESP: 17/05341-1 - Efeito do extrato de chá verde (GTE) rico em catequinas na doença periodontal induzida em ratas ovariectomizadas.
• Beneficiário: Karina Fittipaldi Bombonato Prado
• Modalidade de apoio: Auxílio à Pesquisa - Regular