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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Phosphatidylserine controls calcium phosphate nucleation and growth on lipid monolayers: A physicochemical understanding of matrix vesicle-driven biomineralization

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Autor(es):
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Cruz, Marcos A. E. [1] ; Ferreira, Claudio R. [1] ; Tovani, Camila B. [1] ; de Oliveira, Flavia A. [2] ; Bolean, Mayte [1] ; Caseli, Luciano [3] ; Mebarek, Saida [4] ; Millan, Jose Luis [2] ; Buchet, Rene [4] ; Bottini, Massimo [2, 5] ; Ciancaglini, Pietro [1] ; Ramos, Ana Paula [1]
Número total de Autores: 12
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Dept Quim, FFCLRP, Fac Filosofia Ciencias & Letras Ribeirao Preto, Sao Paulo - Brazil
[2] Sanford Burnham Prebys Med Discovery Inst, La Jolla, CA 92037 - USA
[3] Univ Fed Sao Paulo, Inst Environm Chem & Pharmaceut Sci, Sao Paulo - Brazil
[4] Univ Lyon, UMR 5246, CNRS, ICBMS, Villeurbanne - France
[5] Univ Roma Tor Vergata, Dept Expt Med, I-00133 Rome - Italy
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Journal of Structural Biology; v. 212, n. 2 NOV 1 2020.
Citações Web of Science: 0
Resumo

Bone biomineralization is an exquisite process by which a hierarchically organized mineral matrix is formed. Growing evidence has uncovered the involvement of one class of extracellular vesicles, named matrix vesicles (MVs), in the formation and delivery of the first mineral nuclei to direct collagen mineralization. MVs are released by mineralization-competent cells equipped with a specific biochemical machinery to initiate mineral formation. However, little is known about the mechanisms by which MVs can trigger this process. Here, we present a combination of in situ investigations and ex vivo analysis of MVs extracted from growing-femurs of chicken embryos to investigate the role played by phosphatidylserine (PS) in the formation of mineral nuclei. By using self-assembled Langmuir monolayers, we reconstructed the nucleation core - a PS-enriched motif thought to trigger mineral formation in the lumen of MVs. In situ infrared spectroscopy of Langmuir monolayers and ex situ analysis by transmission electron microscopy evidenced that mineralization was achieved in supersaturated solutions only when PS was present. PS nucleated amorphous calcium phosphate that converted into biomimetic apatite. By using monolayers containing lipids extracted from native MVs, mineral formation was also evidenced in a manner that resembles the artificial PS-enriched monolayers. PS-enrichment in lipid monolayers creates nanodomains for local increase of supersaturation, leading to the nucleation of ACP at the interface through a multistep process. We posited that PS-mediated nucleation could be a predominant mechanism to produce the very first mineral nuclei during MV-driven bone/cartilage biomineralization. (AU)

Processo FAPESP: 14/24249-0 - Associação de proteínas osteogênicas a biominerais e óxidos metálicos dopados com terras-raras: interação com sistemas modelo de membrana
Beneficiário:Camila Bussola Tovani
Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
Processo FAPESP: 17/08892-9 - Superfícies bioativas obtidas a partir de filmes Langmuir-Blodgett e biominerais
Beneficiário:Ana Paula Ramos
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 17/20846-2 - Explorando o papel da matriz lipídico-proteica-mineral na biomineralização óssea: uma abordagem biofísica empregando-se filmes automontados
Beneficiário:Marcos Antonio Eufrásio Cruz
Modalidade de apoio: Bolsas no Brasil - Doutorado