Lipidomics of mesenchymal stem cell differentiation

Autor(es)

da Silva, Camila Gonzaga ^[1] ; de Sa Barretto, Leticia Siqueira ^[1] ; Lo Turco, Edson Guimaraes ^[1] ; Santos, Alex de Lima ^[1] ; Lessio, Camila ^[1] ; Martins Junior, Helio Alves ^[2] ; de Almeida, Fernando Goncalves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Surg, Div Urol, Rua Embau 231 Vila Clementino, BR-04039060 Sao Paulo, SP - Brazil

^[2] SCIEX Ltd, Rua Julio Gonzalez 132, BR-01156060 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemistry and Physics of Lipids; v. 232, OCT 2020.
• Citações Web of Science: 0

Resumo

Mesenchymal stem cells (MSCs), such as adipose-derived stem cells (ADSCs) and skeletal muscle-derived stem cells (MDSCs), are potential sources for cell-based therapeutic strategies. However, there is little knowledge about the lipid composition of these stem cells and the mechanisms of their differentiation. Lipids have important biological and physiological functions that are critical for understanding the regulation and control of stem cell fate. This study sought to analyze the lipidome of rabbit ADSCs and MDSCs and their adipogenic and osteogenic differentiation. The MSCs were isolated and were characterized by flow cytometry. Lipids were extracted from both MSCs and differentiated cells, and the lipids were subsequently analyzed with a hybrid triple quadrupole time-of-flight mass spectrometer. The results showed a total of 1687 lipid species. MSCs exhibited different lipid profiles as well as changes in lipid composition after differentiation. Furthermore, the expression levels of N-acylphosphatidylethanolamine (NAPE) 54:7+NH4 (-FA 17:0(NH4)) and phosphatidylcholine (PC) 42:6+Na were higher in the adipogenic lineages in of both MSC types, and NAPE 58:2+NH4 (-FA 17:0 (NH4)) and NAPE 56:2+NH4 (-FA 17:0 (NH4)) had higher levels in the osteogenic lineages, suggesting lipid similarities in cells differentiated from different stem cell sources. (AU)

• Processo FAPESP: 11/16224-0 - Avaliação do perfil lipídico de células tronco derivadas do tecido adiposo e do tecido muscular de coelhos pré e pós-diferenciação adipogênica e osteogênica
• Beneficiário: Camila Gonzaga da Silva
• Modalidade de apoio: Bolsas no Brasil - Mestrado