Recovering the osteoblastic differentiation potential of mesenchymal stem cells derived from diabetic rats by photobiomodulation therapy

Autor(es)

Bueno, Natalia Pieretti ^[1] ; Copete, Isabella Nunes ^[1] ; Lopes, Helena Bacha ^[2] ; Arany, Praveen R. ^[3] ; Marques, Marcia Martins ^{[1, 4]} ; Ferraz, Emanuela Prado ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Bone Res Lab, Sao Paulo - Brazil

^[3] SUNY Buffalo, Sch Dent Med, New York, NY - USA

^[4] Univ Ibirapuera, Sch Dent, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. 14, n. 3 NOV 2020.
• Citações Web of Science: 0

Resumo

Autologous cell-based therapy for bone regeneration might be impaired by diabetes mellitus (DM) due to the negative effects on mesenchymal stem cells (MSCs) differentiation. Strategies to recover their osteogenic potential could optimize the results. We aimed to evaluate the effect of photobiomodulation (PBM) therapy on osteoblast differentiation of rats with induced DM. Bone marrow MSCs of healthy and diabetic rats were isolated and differentiated into osteoblasts (OB and dOB, respectively). dOB were treated with PBM therapy every 72 hour (660 nm; 0.14 J; 20 mW; 0.714 W/cm(2), and 5 J/cm(2)). Cell morphology, viability, gene and protein expression of osteoblastic markers, alkaline phosphatase (ALP) activity, and the mineralized matrix production of dOB-PBM were compared to dOB. PBM therapy improved viability of dOB, increased the gene and protein expression of bone markers, the ALP activity and the mineralized matrix production. PBM therapy represents an innovative therapeutic approach to optimize the treatment of bone defects in diabetic patients. (AU)

• Processo FAPESP: 18/20302-5 - Efeito da terapia de fotobiomodulação na diferenciação osteoblástica de células-tronco mesenquimais derivadas da medula óssea de ratos diabéticos
• Beneficiário: Isabella Nunes Copete
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica