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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Genomic Organization and Generation of Genetic Variability in the RHS (Retrotransposon Hot Spot) Protein Multigene Family in Trypanosoma cruzi

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Autor(es):
Bernardo, Werica P. [1] ; Souza, Renata T. [1] ; Costa-Martins, Andre G. [2] ; Ferreira, Eden R. [1] ; Mortara, Renato A. [1] ; Teixeira, Marta M. G. [2] ; Ramirez, Jose Luis [3] ; Da Silveira, Jose F. [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, BR-05508000 Sao Paulo, SP - Brazil
[3] Univ Cent Venezuela, Fdn Inst Estudios Avanzados IDEA, Caracas 1080 - Venezuela
Número total de Afiliações: 3
Tipo de documento: Artigo Científico
Fonte: GENES; v. 11, n. 9 SEP 2020.
Citações Web of Science: 0
Resumo

Retrotransposon Hot Spot (RHS) is the most abundant gene family in Trypanosoma cruzi, with unknown function in this parasite. The aim of this work was to shed light on the organization and expression of RHS in T. cruzi. The diversity of the RHS protein family in T. cruzi was demonstrated by phylogenetic and recombination analyses. Transcribed sequences carrying the RHS domain were classified into ten distinct groups of monophyletic origin. We identified numerous recombination events among the RHS and traced the origins of the donors and target sequences. The transcribed RHS genes have a mosaic structure that may contain fragments of different RHS inserted in the target sequence. About 30% of RHS sequences are located in the subtelomere, a region very susceptible to recombination. The evolution of the RHS family has been marked by many events, including gene duplication by unequal mitotic crossing-over, homologous, as well as ectopic recombination, and gene conversion. The expression of RHS was analyzed by immunofluorescence and immunoblotting using anti-RHS antibodies. RHS proteins are evenly distributed in the nuclear region of T. cruzi replicative forms (amastigote and epimastigote), suggesting that they could be involved in the control of the chromatin structure and gene expression, as has been proposed for T. brucei. (AU)

Processo FAPESP: 16/15000-4 - Trypanosoma cruzi: variabilidade genômica intra- e interespecífica e mecanismos de invasão/evasão celular
Beneficiário:Renato Arruda Mortara
Linha de fomento: Auxílio à Pesquisa - Temático
Processo FAPESP: 16/16918-5 - Estudo do egresso de tripomastigotas de Trypanosoma cruzi em células infectadas
Beneficiário:Éden Ramalho de Araujo Ferreira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado