Intranasal Vaccine Using P10 Peptide Complexed within Chitosan Polymeric Nanoparticles as Experimental Therapy for Paracoccidioidomycosis in Murine Model

Autor(es)

Rodrigues Dos Santos Junior, Samuel ^[1] ; Kelley Lopes da Silva, Francenya ^[2] ; Santos Dias, Lucas ^{[1, 3]} ; Oliveira Souza, Ana Camila ^{[1, 4]} ; Valdemir de Araujo, Marcelo ^[1] ; Buffoni Roque da Silva, Leandro ^[1] ; Travassos, Luiz R. ^[5] ; Correa Amaral, Andre ^[2] ; P. Taborda, Carlos ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508000 Sao Paulo, SP - Brazil

^[2] Univ Fed Goias, Inst Patol Trop & Saude Publ, Dept Biotecnol, Lab Nano & Biotecnol, BR-74605050 Goiania, Go - Brazil

^[3] Univ Wisconsin, Dept Pediat, Sch Med & Publ Hlth, Madison, WI 53706 - USA

^[4] Univ Tennessee, Hlth Sci Ctr, Memphis, TN 38163 - USA

^[5] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Inst Med Trop Sao Paulo, Dept Dermatol, Fac Med, BR-05403000 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FUNGI; v. 6, n. 3 SEP 2020.
• Citações Web of Science: 0

Resumo

Paracoccidioidomycosis (PCM) is a granulomatous fungal disease caused by the dimorphic fungal species of Paracoccidioides, which mainly affects the lungs. Modern strategies for the treatment and/or prevention of PCM are based on a Th1-type immune response, which is important for controlling the disease. One of the most studied candidates for a vaccine is the P10 peptide, derived from the 43 kDa glycoprotein of Paracoccidioides brasiliensis. In order to improve its immune modulatory effect, the P10 peptide was associated with a chitosan-conjugated nanoparticle. The nanoparticles presented 220 nm medium size, poly dispersion index (PDI) below 0.5, zeta potential of +20 mV and encapsulation efficiency around 90%. The nanoparticles' non-toxicity was verified by hemolytic test and cell viability using murine macrophages. The nanoparticles were stable and presented physicochemical characteristics desirable for biological applications, reducing the fungal load and the usual standard concentration of the peptide from 4 to 20 times. (AU)

• Processo FAPESP: 16/08730-6 - Patogenicidade fúngica: efeito do tabagismo, resposta imune e a modulação vacinal na paracoccidioidomicose e na histoplasmose
• Beneficiário: Carlos Pelleschi Taborda
• Modalidade de apoio: Auxílio à Pesquisa - Temático