Proteomic and Transcriptomic Analyses Indicate Metabolic Changes and Reduced Defense Responses in Mycorrhizal Roots of Oeceoclades maculata (Orchidaceae) Collected in Nature

Autor(es)

Valadares, Rafael B. S. ^{[1, 2]} ; Perotto, Silvia ^{[3, 4]} ; Lucheta, Adriano R. ^[5] ; Santos, Eder C. ^[6] ; Oliveira, Renato M. ^{[2, 7]} ; Lambais, Marcio R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Ciencia Solo, Ave Padua Dias 11, BR-13418900 Piracicaba - Brazil

^[2] Inst Tecnol Vale, Rua Boaventura da Silva 955, BR-66050000 Belem, Para - Brazil

^[3] Univ Torino, Dipartimento Sci Vita & Biol Sistemi, Viale Mattioli 25, I-10125 Turin - Italy

^[4] IPSP CNR, Viale Mattioli 25, I-10125 Turin - Italy

^[5] SENAI Innovat Inst Mineral Technol, Ave Bras de Aguiar 548, BR-66035405 Belem, Para - Brazil

^[6] Univ Tecnol Fed Parana, BR-85601970 Linha Santa Barbara, Francisco Beltr - Brazil

^[7] Univ Fed Minas Gerais, Inst Ciencias Biol, Ave Pres Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF FUNGI; v. 6, n. 3 SEP 2020.
• Citações Web of Science: 0

Resumo

Orchids form endomycorrhizal associations with fungi mainly belonging to basidiomycetes. The molecular events taking place in orchid mycorrhiza are poorly understood, although the cellular changes necessary to accommodate the fungus and to control nutrient exchanges imply a modulation of gene expression. Here, we used proteomics and transcriptomics to identify changes in the steady-state levels of proteins and transcripts in the roots of the green terrestrial orchidOeceoclades maculata. When mycorrhizal and non-mycorrhizal roots from the same individuals were compared, 94 proteins showed differential accumulation using the label-free protein quantitation approach, 86 using isobaric tagging and 60 using 2D-differential electrophoresis. Afterde novoassembly of transcriptomic data, 11,179 plant transcripts were found to be differentially expressed, and 2175 were successfully annotated. The annotated plant transcripts allowed the identification of up- and down-regulated metabolic pathways. Overall, proteomics and transcriptomics revealed, in mycorrhizal roots, increased levels of transcription factors and nutrient transporters, as well as ethylene-related proteins. The expression pattern of proteins and transcripts involved in plant defense responses suggested that plant defense was reduced inO. maculatamycorrhizal roots sampled in nature. These results expand our current knowledge towards a better understanding of the orchid mycorrhizal symbiosis in adult plants under natural conditions. (AU)

• Processo FAPESP: 11/09637-6 - Desenvolvimento e avaliação de biofertilizantes contendo S0 e bactérias oxidantes de S
• Beneficiário: Adriano Reis Lucheta
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/01727-6 - Identificação e caracterização de proteínas envolvidas na regulação do desenvolvimento de micorrizas em orquídeas
• Beneficiário: Rafael Borges da Silva Valadares
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 10/00939-7 - Metaproteômica microbiana da filosfera da Mata Atlântica
• Beneficiário: Eder da Costa dos Santos
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/50266-4 - Identificacao e caracterizacao de genes e proteinas envolvidas na regulacao do desenvolvimento de micorrizas em orquideas.
• Beneficiário: Fernando Dini Andreote
• Modalidade de apoio: Auxílio à Pesquisa - Regular