| Texto completo | |
| Autor(es): |
Chi, Jordi
[1]
;
Cova, Marta
[1]
;
de las Rivas, Matilde
[2]
;
Medina, Ana
[3]
;
Borges, Rafael Junqueira
[4, 3]
;
Leivar, Pablo
[5]
;
Planas, Antoni
[5]
;
Uson, Isabel
[6, 3]
;
Hurtado-Guerrero, Ramon
[2, 7, 8, 9]
;
Izquierdo, Luis
[1]
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] Univ Barcelona, HospClin, ISGlobal, Barcelona - Spain
[2] Univ Zaragoza, Inst Biocomputat & Phys Complex Syst BIFI, Zaragoza - Spain
[3] Inst Mol Biol Barcelona IBMB CSIC, Crystallog Methods, Barcelona - Spain
[4] Univ Estadual Paulista UNESP, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP - Brazil
[5] Univ Ramon Llull, Inst Quim Sarria, Lab Biochem, Barcelona - Spain
[6] Inst Catalana Recerca & Estudis Avancats, ICREA, Barcelona - Spain
[7] Univ Copenhagen, Copenhagen Ctr Glyc, Sch Dent, Dept Cellular & Mol Med, Copenhagen - Denmark
[8] Univ Zaragoza, Lab Microscopias Avanzada LMA, Zaragoza - Spain
[9] Fdn ARAID, Zaragoza - Spain
Número total de Afiliações: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | MBIO; v. 11, n. 5 SEP-OCT 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
UDP-N-acetylglucosamine (UDP-GlcNAc), the main product of the hexosamine biosynthetic pathway, is an important metabolite in protozoan parasites since its sugar moiety is incorporated into glycosylphosphatidylinositol (GPI) glycolipids and N- and O-linked glycans. Apicomplexan parasites have a hexosamine pathway comparable to other eukaryotic organisms, with the exception of the glucosamine-phosphate N-acetyltransferase (GNA1) enzymatic step that has an independent evolutionary origin and significant differences from nonapicomplexan GNA1s. By using conditional genetic engineering, we demonstrate the requirement of GNA1 for the generation of a pool of UDP-GlcNAc and for the development of intraerythrocytic asexual Plasmodium falciparum parasites. Furthermore, we present the 1.95 A resolution structure of the GNA1 ortholog from Cryptosporidium parvum, an apicomplexan parasite which is a leading cause of diarrhea in developing countries, as a surrogate for P. falciparum GNA1. The indepth analysis of the crystal shows the presence of specific residues relevant for GNA1 enzymatic activity that are further investigated by the creation of site-specific mutants. The experiments reveal distinct features in apicomplexan GNA1 enzymes that could be exploitable for the generation of selective inhibitors against these parasites, by targeting the hexosamine pathway. This work underscores the potential of apicomplexan GNA1 as a drug target against malaria. IMPORTANCE Apicomplexan parasites cause a major burden on global health and economy. The absence of treatments, the emergence of resistances against available therapies, and the parasite's ability to manipulate host cells and evade immune systems highlight the urgent need to characterize new drug targets to treat infections caused by these parasites. We demonstrate that glucosamine-6-phosphate N-acetyltransferase (GNA1), required for the biosynthesis of UDP-N-acetylglucosamine (UDP-GlcNAc), is essential for P. falciparum asexual blood stage development and that the disruption of the gene encoding this enzyme quickly causes the death of the parasite within a life cycle. The high-resolution crystal structure of the GNA1 ortholog from the apicomplexan parasite C. parvum, used here as a surrogate, highlights significant differences from human GNA1. These divergences can be exploited for the design of specific inhibitors against the malaria parasite. (AU) | |
| Processo FAPESP: | 16/24191-8 - Desenvolvimento de métodos para elucidação de estruturas cristalográficas e estudos estruturais do mecanismo tóxico de fosfolipases A2 homólogas de veneno de serpente |
| Beneficiário: | Rafael Junqueira Borges |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |