| Texto completo | |
| Autor(es): |
Cheng, Ryan R.
[1]
;
Contessoto, Vinicius G.
[1, 2]
;
Aiden, Erez Lieberman
[1, 3]
;
Wolynes, Peter G.
[4, 1, 5, 6]
;
Di Pierro, Michele
[1, 7]
;
Onuchic, Jose N.
[4, 1, 5, 6]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Rice Univ, Ctr Theoret Biol Phys, Houston, TX 77005 - USA
[2] Brazilian Ctr Res Energy & Mat CNPEM, Brazilian Biorenewables Natl Lab LNBR, Campinas - Brazil
[3] Baylor Coll Med, Ctr Genome Architecture, Houston, TX 77030 - USA
[4] Rice Univ, Dept Chem, Houston, TX 77005 - USA
[5] Rice Univ, Dept Biosci, Houston, TX 77005 - USA
[6] Rice Univ, Dept Phys & Astron, Houston, TX 77005 - USA
[7] Northeastern Univ, Dept Phys, Boston, MA 02115 - USA
Número total de Afiliações: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | eLIFE; v. 9, OCT 13 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Using computer simulations, we generate cell-specific 3D chromosomal structures and compare them to recently published chromatin structures obtained through microscopy. We demonstrate using machine learning and polymer physics simulations that epigenetic information can be used to predict the structural ensembles of multiple human cell lines. Theory predicts that chromosome structures are fluid and can only be described by an ensemble, which is consistent with the observation that chromosomes exhibit no unique fold. Nevertheless, our analysis of both structures from simulation and microscopy reveals that short segments of chromatin make twostate transitions between closed conformations and open dumbbell conformations. Finally, we study the conformational changes associated with the switching of genomic compartments observed in human cell lines. The formation of genomic compartments resembles hydrophobic collapse in protein folding, with the aggregation of denser and predominantly inactive chromatin driving the positioning of active chromatin toward the surface of individual chromosomal territories. (AU) | |
| Processo FAPESP: | 17/09662-7 - Evolução racional por métodos computacionais aplicada em enzimas relacionadas com a produção de bioetanol |
| Beneficiário: | Vinícius de Godoi Contessoto |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado |
| Processo FAPESP: | 16/13998-8 - Evolução racional por métodos computacionais aplicados na predição de mutações no desenvolvimento de enzimas para produção de biocombustíveis |
| Beneficiário: | Vinícius de Godoi Contessoto |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |