Immunoinformatics and analysis of antigen distribution of Ureaplasma diversum strains isolated from different Brazilian states

Autor(es):Mostrar menos -

Santos Junior, Manoel Neres ^{[1, 2]} ; Santos, Ronaldo Silva ^[1] ; Neves, Wanderson Souza ^[1] ; Fernandes, Janaina Marinho ^[1] ; de Brito Guimaraes, Bruna Carolina ^[2] ; Barbosa, Maysa Santos ^[3] ; Coelho Silva, Lucas Santana ^[2] ; Gomes, Camila Pacheco ^[2] ; Rezende, Izadora Souza ^[3] ; Teixeira Oliveira, Caline Novaes ^{[1, 2]} ; de Macedo Neres, Nayara Silva ^[1] ; Campos, Guilherme Barreto ^[1] ; Bastos, Bruno Lopes ^[1] ; Timenetsky, Jorge ^[3] ; Marques, Lucas Miranda ^{[3, 1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Bahia, Multidisciplinary Inst Hlth, Dept Biointeract, Rua Hormindo Barros, 58 Quadra 17 Lote 58, BR-45029094 Vitoria Da Conquista, BA - Brazil

^[2] State Univ Santa Cruz UESC, Dept Microbiol, Ilheus, BA - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC Veterinary Research; v. 16, n. 1 OCT 7 2020.
• Citações Web of Science: 0

Resumo

BackgroundUreaplasma diversum has numerous virulence factors that contribute to pathogenesis in cattle, including Lipid-associated membrane proteins (LAMPs). Therefore, the objectives of this study were to evaluate in silico important characteristics for immunobiological applications and for heterologous expression of 36 LAMPs of U. diversum (UdLAMPs) and, also, to verify by conventional PCR the distribution of these antigens in strains of Brazilian states (Bahia, Minas Gerais, SAo Paulo, and Mato Grosso do Sul). The Manatee database was used to obtain the gene and peptide sequences of the antigens. Similarity and identity studies were performed using BLASTp and direct antigenicity was evaluated by the VaxiJen v2.0 server. Epitope prediction for B lymphocytes was performed on the BepiPred v2.0 and CBTOPE v1.0 servers. NetBoLApan v1.0 was used to predict CD8(+) T lymphocyte epitopes. Subcellular location and presence of transmembrane regions were verified by the software PSORTb v3.0.2 and TMHMM v2.2 respectively. SignalP v5.0, SecretomeP v2.0, and DOLOP servers were used to predict the extracellular excretion signal. Physico-chemical properties were evaluated by the web-software ProtParam, Solpro, and Protein-sol.ResultsIn silico analysis revealed that many UdLAMPs have desirable properties for immunobiological applications and heterologous expression. The proteins gudiv\_61, gudiv\_103, gudiv\_517, and gudiv\_681 were most promising. Strains from the 4 states were PCR positive for antigens predicted with immunogenic and/or with good characteristics for expression in a heterologous system.ConclusionThese works contribute to a better understanding of the immunobiological properties of the UdLAMPs and provide a profile of the distribution of these antigens in different Brazilian states. (AU)

• Processo FAPESP: 17/25686-3 - Desenvolvimento de teste de diagnóstico e seleção de candidatos vacinais para micoplasmose em bovinos e caprinos
• Beneficiário: Jorge Timenetsky
• Modalidade de apoio: Auxílio à Pesquisa - Regular