Combined isoflavones biotransformation increases the bioactive and antioxidant capacity of soymilk

Autor(es)

de Queiros, Livia Dias ^[1] ; de Avila, Amanda Rejane Alves ^[1] ; Botaro, Andressa Vianna ^[1] ; Chirotto, Danielle Branta Lopes ^[1] ; Macedo, Juliana Alves ^[1] ; Macedo, Gabriela Alves ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food & Nutr, Rua Monteiro Lobato, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Applied Microbiology and Biotechnology; v. 104, n. 23, p. 10019-10031, DEC 2020.
• Citações Web of Science: 1

Resumo

Isoflavones are phenolic secondary metabolites mainly occurring in soy and soybean products. Compared to glycoside forms, isoflavone aglycones present higher biological activities. This study evaluated the potential of microbial and enzymatic treatments in biotransformed isoflavones in their biologically active forms in soymilk. Seven different cultures of lactic acid bacteria and bifidobacteria associated with the action of immobilized tannase enzyme were screened for isoflavone glycoside biotransformation ability. The biotransformed soymilk samples were characterized regarding isoflavone profile, total phenolic content, and in vitro antioxidant activities. All bacterial strains showed a good growth capacity in soymilk matrix and produced beta-glucosidase enzyme, which hydrolyzed isoflavone glycosides into aglycones in soymilk after 24 h of fermentation. The microbial fermentation followed by tannase reaction (FT processes) resulted in the highest increase of bioactive aglycones (10.3- to 13.1-fold for daidzein, 10.4- to 12.3-fold for genistein, and 3.8- to 4.7-fold for glycitein), compared to control soymilk. Further, FT processes enhanced the total phenolic content (53-70%) and antioxidant activity by ORAC (69-102%) and FRAP (49-71%) assays of the soymilk matrix. Therefore, the combination of microbial fermentation and tannase treatment is a promising strategy to obtain a fermented soy product rich in bioactive isoflavones with greater health-promoting potential. (AU)

• Processo FAPESP: 16/20965-9 - Biotransformação de isoflavonas de soja para obtenção de produto rico em compostos bioativos e caráter probiótico
• Beneficiário: Lívia Dias de Queirós
• Modalidade de apoio: Bolsas no Brasil - Doutorado