Inhibiting diacylglycerol acyltransferase-1 reduces lipid biosynthesis in bovine blastocysts produced in vitro

Autor(es)

Canon-Beltran, K. ^{[1, 2]} ; Giraldo-Giraldo, J. ^{[1, 3]} ; Cajas, Y. N. ^[1] ; Beltran-Brena, P. ^[1] ; Hidalgo, C. O. ^[4] ; Vasquez, N. ^[3] ; Leal, C. L. V. ^{[5, 1]} ; Gutierrez-Adan, A. ^[1] ; Gonzalez, E. M. ^[6] ; Rizos, D. ^[1]

Afiliação do(s) autor(es)

^[1] Natl Inst Agr & Food Res & Technol INIA, Dept Anim Reprod, Ctra Coruna KM 5, 9, Madrid 28040 - Spain

^[2] Univ Tecn Particular Loja, Dept Ciencias Biol, Loja - Ecuador

^[3] Univ Nacl Colombia, Sch Biosci, Fac Sci, Reprod Biotechnol Lab, Medellin - Colombia

^[4] Reg Agrifood Res & Dev Serv Asturias SERIDA, Dept Anim Select & Reprod, Gijon - Spain

^[5] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Pirassununga - Brazil

^[6] Complutense Univ Madrid UCM, Fac Vet, Dept Anat & Embryol, Madrid - Spain

• Tipo de documento: Artigo Científico
• Fonte: Theriogenology; v. 158, p. 267-276, DEC 2020.
• Citações Web of Science: 0

Resumo

Diacylglycerol acyltransferase-1 (DGAT1) is one of the DGAT enzymes that catalyzes the final step in the synthesis of triacylglycerol, which is a major component of the lipid droplets in embryos. Intracellular lipids accumulated in embryos produced in vitro have been associated with reduced cryotolerance and quality. The objective of the present study was to investigate the influence of DGAT1 inhibition on embryo development, quality, and post-vitrification survival, in addition to expression profiles of selected lipid metabolism-regulating and oxidative stress genes. Bovine cumulus-oocyte complexes were matured and fertilized in vitro and were cultured in synthetic oviduct fluid (SOF) supplemented with 5% fetal calf serum (FCS) alone (Control) or with 1, 5, 10 or 50 mu M DGAT1 inhibitor (A922500(R); D1, D5, D10, and D50, respectively) or 0.1% dimethyl sulfoxide (CDMSO: vehicle for DGAT1 inhibitor dilution) from 54 h post-insemination until Day 8 post insemination. No differences were found in blastocyst yield on days 7 and 8 in Control, CDMSO, D10, and D50 groups. Embryos cultured with 10 or 50 mu M DGAT1 inhibitor had greater mitochondrial activity (P < 0.01), and increased number of cells (P < 0.05), while the cytoplasmic lipid content was reduced (P < 0.01), the latter associated with altered expression profiles of selected genes regulating lipid metabolism or genes related with oxidative stress (transcript abundance increased for SLC2A1 and SLC2A5 and decreased for DGAT1 and GPX1). Importantly, the survival rate of blastocysts produced with 10 mu M DGAT1 was higher than that of Control, CDMSO and D50 groups at 72 h after vitrification and warming (73.8 vs 57.1, 55.9 and 56.1%, respectively, P < 0.001). In conclusion, inhibition of DGAT1 synthesis in bovine embryos produced in vitro abrogates the negative effect of FCS by decreasing their lipid content, increasing mitochondria activity and improving embryo cryotolerance, as well as favoring the expression of lipid metabolism regulating and oxidative stress-related transcripts. (C) 2020 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 17/20339-3 - Influência de vesículas extracelulares no metabolismo lipídico e no desenvolvimento e qualidade de embriões bovinos produzidos in vitro
• Beneficiário: Cláudia Lima Verde Leal
• Modalidade de apoio: Bolsas no Exterior - Pesquisa