Extracellular biofilm matrix leads to microbial dysbiosis and reduces biofilm susceptibility to antimicrobials on titanium biomaterial: An in vitro and in situ study

Autor(es)

Costa, Raphael Cavalcante ^[1] ; Souza, Joao Gabriel Silva ^[1] ; Bertolini, Martinna ^[2] ; Retamal-Valdes, Belen ^[3] ; Feres, Magda ^[3] ; Barao, Valentim A. R. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Piracicaba Dent Sch, Dept Prosthodont & Periodontol, Ave Limeira 901, BR-13414903 Piracicaba, SP - Brazil

^[2] Univ Connecticut UCONN, Sch Dent Med, Farmington, CT - USA

^[3] Guarulhos Univ UNG, Dent Res Div, Dept Periodontol, Guarulhos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Clinical Oral Implants Research; v. 31, n. 12, p. 1173-1186, DEC 2020.
• Citações Web of Science: 0

Resumo

Objectives: To test the role of exopolysaccharide (EPS) polymers matrix to modulate the composition/virulence of biofilms growing on titanium (Ti) surfaces, the effect on antibiotic susceptibility, and whether a dual-targeting therapy approach for disrupted EPS matrix could improve the antimicrobial effect. Materials and Methods: A microcosm biofilm model using human saliva as inoculum was used, and the microbial composition was assessed by checkerboard DNA-DNA hybridization. EPS-enriched biofilms virulence was tested using fibroblast monolayer. Povidone-iodine (PI) was used as EPS-targeting agent followed by amoxicillin + metronidazole antibiotic to reduce bacterial biomass using an in situ model. Results: An EPS-enriched environment, obtained by sucrose exposure, promoted bacterial accumulation and led to a dysbiosis on biofilms, favoring the growth ofStreptococcus, Fusobacterium,andCampylobacterspecies and even strict anaerobic species related to peri-implant infections, such asPorphyromonas gingivalisandTannerella forsythia(similar to 3-fold increase). EPS-enriched biofilm transitioned from a commensal aerobic to a pathogenic anaerobic profile. EPS increased biofilm virulence promoting higher host cell damage and reduced antimicrobial susceptibility, but the use of a dual-targeting approach with PI pre-treatment disrupted EPS matrix scaffold, increasing antibiotic effect on in situ biofilms. Conclusion: Altogether, our data provide new insights of how EPS matrix creates an environment that favors putative pathogens growth and shed light to a promising approach that uses matrix disruption as initial step to potentially improve implant-related infections treatment. (AU)

• Processo FAPESP: 18/04630-2 - Síntese de filme de vidro bioativo para superfície de titânio por meio de plasma eletrolítico de oxidação: análise eletroquímica e antimicrobiana em modelo de microcosmos
• Beneficiário: Raphael Cavalcante Costa
• Modalidade de apoio: Bolsas no Brasil - Mestrado