Aptamers recognizing fibronectin confer improved bioactivity to biomaterials and promote new bone formation in a periodontal defect in rats

Autor(es)

Parisi, Ludovica ^{[1, 2, 3]} ; Rivara, Federico ^{[1, 2]} ; Costa, Camila A. ^{[4, 5]} ; Abuna, Rodriguo P. F. ^{[6, 7]} ; Palioto, Daniela B. ^[4] ; Macaluso, Guido M. ^{[1, 2, 8]}

Afiliação do(s) autor(es)

^[1] Univ Parma, Ctr Univ Odontoiatria, Via Gramsci 14, I-43126 Parma - Italy

^[2] Univ Parma, Dipartimento Med & Chirurg, Via Gramsci 14, I-43126 Parma - Italy

^[3] Univ Bern, Sch Dent Med, Dept Orthodont & Dentofacial Orthoped, Lab Oral Mol Biol, Dent Res Ctr, Freiburgstr 3, CH-3010 Bern - Switzerland

^[4] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Av Cafe, BR-14040904 Ribeirao Preto, SP - Brazil

^[5] Univ Fed Goias, Sch Dent, Dept Stomatol Sci, Ave Aruma, BR-74835320 Goiania, Go - Brazil

^[6] Univ Sao Paulo, Sch Dent Ribeirao Preto, Cell Culture Lab, Av Cafe, BR-14040904 Ribeirao Preto, SP - Brazil

^[7] Univ Sao Paulo, Fiocruz Biinst Translat Med Project, Dept Biochem & Immunol, Ribeirao Preto Med Sch, Rua Tecn, BR-14040904 Ribeirao Preto, SP - Brazil

^[8] CNR, Ist Mat Elettron & Il Magnetismo, Parco Area Sci 37-A, I-43124 Parma - Italy

• Tipo de documento: Artigo Científico
• Fonte: Biomedical Materials; v. 16, n. 1 JAN 1 2021.
• Citações Web of Science: 0

Resumo

The use of alloplastic materials in periodontal regenerative therapies is limited by their incapacity to establish a dynamic dialog with the surrounding milieu. The aim of the present study was to control biomaterial surface bioactivity by introducing aptamers to induce the selective adsorption of fibronectin from blood, thus promoting platelets activation in vitro and bone regeneration in vivo. A hyaluronic acid/polyethyleneglycole-based hydrogel was enriched with aptamers selected for recognizing and binding fibronectin. In vitro, the capacity of constructs to support osteoblast adhesion, as well as platelets aggregation and activation was assessed by chemiluminescence within 24 h. Matrices were then evaluated in a rat periodontal defect to assess their regenerative potential by microcomputed tomography (mu CT) and their osteogenic capacity by Luminex assay 5, 15 and 30 d postoperatively. Aptamers were found to confer matrices the capacity of sustaining firm cell adhesion (p = 0.0377) and to promote platelets activation (p = 0.0442). In vivo, aptamers promoted new bone formation 30 d post-operatively (p < 0.001) by enhancing osteoblastic lineage commitment maturation. Aptamers are a viable surface modification, which confers alloplastic materials the potential capacity to orchestrate blood clot formation, thus controlling bone healing. (AU)

• Processo FAPESP: 14/03516-0 - Medula óssea e células tronco na regeneração periodontal: análises microtomográfica, histomorfométrica, imunohistoquímica e imunoenzimática em defeitos periodontais de fenestração em ratos
• Beneficiário: Michel Reis Messora
• Modalidade de apoio: Auxílio à Pesquisa - Regular