| Texto completo | |
| Autor(es): |
Lima, Maira de Assis
[1]
;
da Silva, Suely Vieira
[1]
;
Serrano-Garrido, Orlando
[2]
;
Huelsemann, Maren
[3, 4]
;
Santos-Neres, Luana
[1]
;
Carlos Rodriguez-Manzaneque, Juan
[2]
;
Hodgson, Louis
[3, 4]
;
Freitas, Vanessa M.
[1]
Número total de Autores: 8
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Av Prof Lineu Prestes 1524, Ed Biomed 1 Sala 428, BR-05508000 Sao Paulo, SP - Brazil
[2] GENYO, Ctr Genom & Oncol Res, Ave Ilustrac 114, Granada 18016 - Spain
[3] Albert Einstein Coll Med, Dept Anat & Struct Biol, Bronx, NY 10461 - USA
[4] Albert Einstein Coll Med, Gruss Lipper Biophoton Ctr, Bronx, NY 10461 - USA
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CELLULAR SIGNALLING; v. 77, JAN 2021. |
| Citações Web of Science: | 1 |
| Resumo | |
ADAMTSs (A Disintegrin And Metalloproteinase with ThromboSpondin motifs) are secreted proteases dependent on Zn2+/Ca2+, involved in physiological and pathological processes and are part of the extracellular matrix (ECM). Here, we investigated if ADAMTS-1 is required for invasion and migration of cells and the possible mechanism involved. In order to test ADAMTS-1's role in ovarian cancer cells (CHO, NIH-OVCAR-3 and ES2) and NIH-3 T3 fibroblasts, we modified the levels of ADAMTS-1 and compared those to parental. Cells exposed to ADAMTS-1-enriched medium exhibited a decline in cell migration and invasion when compared to controls with or without a functional metalloproteinase domain. The opposite was observed in cells when ADAMTS-1 was deleted via the CRISPR/Cas9 approach. The decline in ADAMTS-1 levels enhanced the phosphorylated form of Src and FAX. We also evaluated the activities of cellular Rho GTPases from cell lysates using the GLISA (R) kit. The Cdc42-GTP signal was significantly increased in the CRISPR ADAMTS-1 ES-2 cells. By a Fodrster resonance energy transfer (FRET) biosensor for Cdc42 activity in ES-2 cells we demonstrated that Cdc42 activity was strongly polarized at the leading edge of migrating cells with ADAMTS-1 deletion, compared to the wild type cells. As conclusion, ADAMTS-1 inhibits proliferation, polarization and migration. (AU) | |
| Processo FAPESP: | 15/19773-5 - Função da progesterona e da protease ADAMTS 1 na migração de células derivadas de câncer de ovário |
| Beneficiário: | Maíra de Assis Lima |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |
| Processo FAPESP: | 18/19813-5 - Função da progesterona e da protease ADAMTS 1 na migração de células derivadas de câncer de ovário |
| Beneficiário: | Maíra de Assis Lima |
| Modalidade de apoio: | Bolsas no Exterior - Estágio de Pesquisa - Doutorado |
| Processo FAPESP: | 18/05566-6 - ADAMTS-1 nuclear regulando o comportamento de células normais e neoplásicas |
| Beneficiário: | Vanessa Morais Freitas |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |