Time domain NMR spectroscopy as a fast method for probing the efficiency of biomass pretreatments for second generation ethanol production

Autor(es)

Bianquini, Tatiane S. ^[1] ; Florencio, Camila ^[2] ; dos Santos Garcia, Rodrigo Henrique ^[3] ; Colnago, Luiz Alberto ^[2] ; Ceccato-Antonini, Sandra R. ^[4] ; Carmo, Marcelo ^[5] ; Teles, Joao ^[1] ; Farinas, Cristiane S. ^{[6, 2]} ; Ribeiro, Tatiana S. ^{[5, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Nat Sci Math & Educ, Rodovia Anhanguera, Km 174 SP-330, BR-13600970 Araras, SP - Brazil

^[2] Embrapa Instrumentacao, Rua 15 Novembro 1452, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Sao Carlos Inst Chem, Av Trabalhador Sao Carlene 400, Caixa Postal 780, BR-780 Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Carlos, Dept Agroind Technol & Rural Socioecon, Rodovia Anhanguera, Km 174, BR-13600970 Araras, SP - Brazil

^[5] Forschungszentrum Julich, Inst Energy & Climate Res IEK 14, Electrochem Proc Engn, D-52425 Julich - Germany

^[6] Univ Fed Sao Carlos, Grad Program Chem Engn, BR-13565905 Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMASS & BIOENERGY; v. 142, NOV 2020.
• Citações Web of Science: 1

Resumo

Herein we describe a simple and fast spectroscopy method based on the Time Domain Nuclear Magnetic Resonance (TD-NMR) method (or low field NMR) to determine the efficiency of lignocellulosic biomass pretreatments for increasing saccharification by enzymes, a key step in second generation (2G) ethanol production. Three different pretreatments (liquid hot water (LHW), steam explosion (SE) and steam-exploded and delignified (SED) process) were used as characteristic processes to reduce biomass recalcitrance, being the untreated and treated biomass (sugarcane bagasse) fully characterized before and after the treatments - using electron microscopy, structural techniques and the direct evaluation of commercial enzyme activity. The analyses in TD-NMR indicate that the binding mode of water (1. Shorter T-2-water molecules of low mobility, which are adsorbed into the cell wall and around the lumens; 2. Intermediate T-2-looser water molecules and within small cellulose pores; 3. Longer T-2-water inside larger cellulose pores and excess water outside the cellulose pores) as evinced by the technique are directly related to the ability of each process for increasing the enzymatic action in the saccharification of pretreated biomass. Given its low cost and fast acquisition time, the proposed method based in TD-NMR could be especially useful as a predictive technique for control routines in large-scale industrial production of 2G ethanol. (AU)

• Processo FAPESP: 15/14306-0 - Uso da Ressonância Magnética Nuclear para investigar materiais lignocelulósicos de bagaço de cana-de-açúcar em pré-tratamentos e na sua hidrólise para a produção de etanol G2
• Beneficiário: Tatiane Bianquini de Godoy
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 16/10636-8 - Da fábrica celular à biorrefinaria integrada Biodiesel-Bioetanol: uma abordagem sistêmica aplicada a problemas complexos em micro e macroescalas
• Beneficiário: Roberto de Campos Giordano
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático