| Texto completo | |
| Autor(es): |
Sallai, Roberto Carlos
[1, 2]
;
Salu, Bruno Ramos
[1]
;
Silva-Lucca, Rosemeire Aparecida
[3]
;
Alves, Flivio Lopes
[1]
;
Napoleao, Thiago Henrique
[4]
;
Guedes Paiva, Patricia Maria
[4]
;
Ferreira, Rodrigo da Silva
[1]
;
Sampaio, Misako Uemura
[1]
;
Vilela Oliva, Maria Luiza
[1]
Número total de Autores: 9
|
| Afiliação do(s) autor(es): | [1] Univ Fed Sao Paulo, Dept Bioquim, Escola Paulista Med, Rua Tres de Maio 100, BR-04044020 Sao Paulo, SP - Brazil
[2] Ctr Univ Fundacao Santo Andre, Av Principe Gales 821, BR-09060650 Santo Andre, SP - Brazil
[3] Univ Estadual Oeste Parana, Ctr Engn & Ciencias Exatas, Unioeste, Campus Toledo, R Fac 645, BR-85903000 Toledo - Spain
[4] Univ Fed Pernambuco, Dept Bioquim, Av Moraes Rego S-N, Cidade Univ, BR-50670420 Recife, PE - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLANTS-BASEL; v. 9, n. 12 DEC 2020. |
| Citações Web of Science: | 0 |
| Resumo | |
Protease inhibitors are involved in the regulation of endogenous cysteine proteases during seed development and play a defensive role because of their ability to inhibit exogenous proteases such as those present in the digestive tracts of insects. Araucaria angustifolia seeds, which can be used in human and animal feed, were investigated for their potential for the development of agricultural biotechnology and in the field of human health. In the pine nuts extract, which blocked the activities of cysteine proteases, it was detected potent insecticidal activity against termites (Nasutitermes corniger) belonging to the most abundant termite genus in tropical regions. The cysteine inhibitor (AaCI-2S) was purified by ion-exchange, size exclusion, and reversed-phase chromatography. Its functional and structural stability was confirmed by spectroscopic and circular dichroism studies, and by detection of inhibitory activity at different temperatures and pH values. Besides having activity on cysteine proteases from C. maculatus digestive tract, AaCI-2S inhibited papain, bromelain, ficin, and cathepsin L and impaired cell proliferation in gastric and prostate cancer cell lines. These properties qualify A. angustifolia seeds as a protein source with value properties of natural insecticide and to contain a protease inhibitor with the potential to be a bioactive molecule on different cancer cells. (AU) | |
| Processo FAPESP: | 17/07972-9 - Desenvolvimento de compostos para profilaxia e tratamento de doenças cardiovasculares |
| Beneficiário: | Maria Luiza Vilela Oliva |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/06630-7 - Fragmentos derivados de proteínas com seletividade para inibição de enzimas de mamíferos e micro-organismos e seu papel como agente anti-inflamatório, antimicrobiano, antitrombótico e antitumoral: mecanismo de ação |
| Beneficiário: | Maria Luiza Vilela Oliva |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |