| Texto completo | |
| Autor(es): |
Brito, Maira Morales
[1]
;
Ramos Angrimani, Daniel de Souza
[1]
;
Rui, Bruno Rogerio
[1]
;
Vechiato Kawai, Giulia Kiyomi
[1]
;
Agostini Losano, Joao Diego
[1]
;
Vannucchi, Camila Infantosi
[1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Reprod, Rua Prof Orlando Marques de Paiva 87, BR-05508270 Sao Paulo - Brazil
Número total de Afiliações: 1
|
| Tipo de documento: | Artigo Científico |
| Fonte: | AGING MALE; v. 23, n. 4, p. 279-286, OCT 1 2020. |
| Citações Web of Science: | 6 |
| Resumo | |
The present research aimed to compare the hormonal profile, sperm quality and freezability of young and senile dogs. Dogs were assigned into Young Group (n = 11) and Senile Group (n = 11), additionally divided into Fresh Semen Group and Cryopreserved Semen Group. Males were evaluated for libido score and blood estrogen and testosterone assay. Sperm morphofunctional evaluations were performed based on Computer Assisted Sperm Analysis, morphology, mitochondrial activity, mitochondrial membrane potential, plasma and acrosomal membrane integrity, and DNA fragmentation. Sperm oxidative features were: protein oxidation, lipid peroxidation and production of advanced glycation end-products. Young dogs had higher libido score, sperm velocity average pathway, linearity of motility and mitochondrial activity index and lower percentage of major defects, total defects and proximal cytoplasmic droplet, despite the lack of difference between hormone profile of aged dogs. Fresh semen of senile dogs had increased percentage of spermatozoa with high mitochondrial membrane potential compared to young dogs and to cryopreserved sperm. Cryopreserved semen of young dogs had higher acrosomal membrane integrity compared to the Senile Group. In conclusion, sperm of aged dogs have reduced quality, signaled by higher morphological defects, ultimately altering sperm mitochondrial function and sperm kinetics. Furthermore, spermatozoa from senile dogs are more sensible to cryoinjury. (AU) | |
| Processo FAPESP: | 15/05419-5 - Senescência reprodutiva em cães: aspectos clínicos e biotecnológicos |
| Beneficiário: | Camila Infantosi Vannucchi |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |