Dynamics of microbial stress responses driven by abiotic changes along a temporal gradient in Deception Island, Maritime Antarctica

Autor(es)

Centurion, V. B. ^{[1, 2]} ; Lacerda-Junior, V, G. ; Duarte, A. W. F. ^{[3, 4]} ; Silva, T. R. ^[4] ; Silva, L. J. ^[5] ; Rosa, L. H. ^[6] ; Oliveira, V. M. ^[4]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Paulinia, SP - Brazil

^[2] State Univ Campinas UNICAMP, Biol Inst, BR-13083862 Campinas, SP - Brazil

^[3] Univ Fed Alagoas, Campus Arapiraca UFAL, BR-57309005 Arapiraca, AL - Brazil

^[4] Lacerda-Junior, G., V, State Univ Campinas UNICAMP, Res Ctr Chem Biol & Agr CPQBA, Microbial Resources Div, BR-13081970 Paulinia, SP - Brazil

^[5] Lacerda-Junior, G., V, Brazilian Agr Res Corp EMBRAPA, BR-13820000 Jaguariuna, SP - Brazil

^[6] Fed Univ Minas Gerais UFMG, Inst Biol Sci, BR-31270901 Belo Horizonte, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Science of The Total Environment; v. 758, MAR 1 2021.
• Citações Web of Science: 0

Resumo

Whalers Bay (WB), Deception Island, is an environment that can drastically change its temperature within a few meters. The main forms of life inhabiting this environment are microorganisms, which, due to the high diversity and their adaptive potential, can survive and thrive under harsh stress conditions. However, the genetic potential and mechanisms to cope with fluctuating adverse conditions as well as what extent environmental variations shape the microbial community over the years it is still unknown in Antarctic environments. In this work, sediments collected in a transect in Whalers Bay, Deception Island, during the Austral Summers of 2014, 2015 and 2017 were analyzed using shotgun metagenomics. Sequence data were further processed with the SqueezeMeta tool for assembly, gene prediction, mapping, taxonomic and functional annotations. Results showed that stress-related functions had the influence of temperatures and solar radiation observed in the years of 2015 and 2017. The most differentiated functions were the ones related to oxidative stress, comparing 2014 vs 2015 and 2014 vs 2017. The genes coding for HSP20 and oxidoreductases (nrdH, grxA, korC and korD), as well as the genes clpE, cspL, and operons mtrAB and vicKR, were differentially enriched between the years, most of them found in gram-positive bacteria. The selective pressures of temperature and radiation may have favored the growth of gram-positive bacteria in 2017, with emphasis on Arthrobacter genus. Data gathered in this work showed that temperature and solar radiation could potentially be the primary driving forces shaping the repertoire of stress-response genes for the maintenance of microbial diversity in WB Antarctic sediments. (C) 2020 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 16/05640-6 - Multi-ômicas aplicadas ao conhecimento e exploração de microbiomas Antárticos
• Beneficiário: Valeria Maia Merzel
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/03172-8 - Mecanismos de adaptação ao estresse em microbioma de sedimentos da Ilha Vulcânica Deception na Antártica marítima
• Beneficiário: Victor Borin Centurion Biruel
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto