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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface

Texto completo
Autor(es):
Oliveira, Viviane C. [1, 2] ; Macedo, Ana P. [2] ; Melo, Luis D. R. [3] ; Santos, Silvio B. [3] ; Hermann, Paula R. S. [1, 4] ; Silva-Lovato, Claudia H. [2] ; Paranhos, Helena F. O. [2] ; Andrade, Denise [1] ; Watanabe, Evandro [1, 5]
Número total de Autores: 9
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Sch Nursing Ribeirao Preto, Human Exposome & Infect Dis Network HEID, Bandeirantes Ave 3900, BR-14040904 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prostheses, Cafe Ave S-N, BR-14040904 Sao Paulo - Brazil
[3] Univ Minho, Ctr Biol Engn CEB, P-4710057 Braga - Portugal
[4] Univ Brasilia, Dept Nursing, BR-72220275 Brasilia, DF - Brazil
[5] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Cafe Ave S-N, BR-14040904 Sao Paulo - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: ANTIBIOTICS-BASEL; v. 10, n. 1 JAN 2021.
Citações Web of Science: 0
Resumo

Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail's ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm. (AU)

Processo FAPESP: 18/09757-0 - Bacteriófagos com potencial aplicabilidade em tubos endotraqueais: atividades antibacteriana e antibiofilme
Beneficiário:Evandro Watanabe
Modalidade de apoio: Auxílio à Pesquisa - Regular
Processo FAPESP: 20/03405-5 - Bacteriófagos com potencial aplicabilidade em tubos endotraqueais: atividades antibacteriana e antibiofilme
Beneficiário:Pedro Castania Amadio Domingues
Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
Processo FAPESP: 19/13271-9 - Bacteriófagos com potencial aplicabilidade em tubos endotraqueais: atividades antibacteriana e antibiofilme
Beneficiário:Felipe Lazarini Bim
Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico