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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Bacteriophage Cocktail-Mediated Inhibition of Pseudomonas aeruginosa Biofilm on Endotracheal Tube Surface

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Author(s):
Oliveira, Viviane C. [1, 2] ; Macedo, Ana P. [2] ; Melo, Luis D. R. [3] ; Santos, Silvio B. [3] ; Hermann, Paula R. S. [1, 4] ; Silva-Lovato, Claudia H. [2] ; Paranhos, Helena F. O. [2] ; Andrade, Denise [1] ; Watanabe, Evandro [1, 5]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Nursing Ribeirao Preto, Human Exposome & Infect Dis Network HEID, Bandeirantes Ave 3900, BR-14040904 Sao Paulo - Brazil
[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prostheses, Cafe Ave S-N, BR-14040904 Sao Paulo - Brazil
[3] Univ Minho, Ctr Biol Engn CEB, P-4710057 Braga - Portugal
[4] Univ Brasilia, Dept Nursing, BR-72220275 Brasilia, DF - Brazil
[5] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Restorat Dent, Cafe Ave S-N, BR-14040904 Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: ANTIBIOTICS-BASEL; v. 10, n. 1 JAN 2021.
Web of Science Citations: 0
Abstract

Although different strategies to control biofilm formation on endotracheal tubes have been proposed, there are scarce scientific data on applying phages for both removing and preventing Pseudomonas aeruginosa biofilms on the device surface. Here, the anti-biofilm capacity of five bacteriophages was evaluated by a high content screening assay. We observed that biofilms were significantly reduced after phage treatment, especially in multidrug-resistant strains. Considering the anti-biofilm screens, two phages were selected as cocktail components, and the cocktail's ability to prevent colonization of the endotracheal tube surface was tested in a dynamic biofilm model. Phage-coated tubes were challenged with different P. aeruginosa strains. The biofilm growth was monitored from 24 to 168 h by colony forming unit counting, metabolic activity assessment, and biofilm morphology observation. The phage cocktail promoted differences of bacterial colonization; nonetheless, the action was strain dependent. Phage cocktail coating did not promote substantial changes in metabolic activity. Scanning electron microscopy revealed a higher concentration of biofilm cells in control, while tower-like structures could be observed on phage cocktail-coated tubes. These results demonstrate that with the development of new coating strategies, phage therapy has potential in controlling the endotracheal tube-associated biofilm. (AU)

FAPESP's process: 18/09757-0 - Potential applicability of bacteriophages on endotracheal tubes: antibacterial and antibiofilm activities
Grantee:Evandro Watanabe
Support Opportunities: Regular Research Grants
FAPESP's process: 20/03405-5 - Potential applicability of bacteriophages on endotracheal tubes: antibacterial and antibiofilm activities
Grantee:Pedro Castania Amadio Domingues
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
FAPESP's process: 19/13271-9 - Potential applicability of bacteriophages on endotracheal tubes: antibacterial and antibiofilm activities
Grantee:Felipe Lazarini Bim
Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training