Comparative Proteomic Analysis of Rhipicephalus sanguineus sensu lato (Acari: Ixodidae) Tropical and Temperate Lineages: Uncovering Differences During Ehrlichia canis Infection

Autor(es):Mostrar menos -

Sanches, Gustavo Seron ^{[1, 2]} ; Villar, Margarita ^{[3, 4, 5]} ; Couto, Joana ^[1] ; Ferrolho, Joana ^[1] ; Fernandez de Mera, Isabel G. ^[3] ; Andr, Marcos Rogerio ^[6] ; Barros-Battesti, Darci Moraes ^[6] ; Machado, Rosangela Zacarias ^[6] ; Bechara, Gervasio Henrique ^[2] ; Mateos-Hernandez, Lourdes ^{[3, 7]} ; de la Fuente, Jose ^{[8, 3]} ; Antunes, Sandra ^[1] ; Domingos, Ana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Nova Lisboa GHTM IHMT UNL, Inst Higiene & Med Trop, Global Hlth & Trop Med, Lisbon - Portugal

^[2] Pontiffaa Univ Catolica Parana, Escola Ciencias Vida, Curitiba, Parana - Brazil

^[3] Inst Invest Recursos Cineget IREC CSIC UCLM JCCM, SaBio, Ciudad Real - Spain

^[4] Univ Castilla La Mancha, Fac Sci & Chem Technol, Biochem Sect, Ciudad Real - Spain

^[5] Univ Castilla La Mancha, Reg Ctr Biomed Res CRIB, Ciudad Real - Spain

^[6] Univ Estadual Paulista FCAV UNESP, Dept Patol Vet, Jaboticabal - Brazil

^[7] Univ Paris Est, ANSES, Ecole Natl Vet Alfort, UMR BIPAR, INRAE, Paris - France

^[8] Oklahoma State Univ, Ctr Vet Hlth Sci, Dept Vet Pathobiol, Stillwater, OK 74078 - USA

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY; v. 10, JAN 29 2021.
• Citações Web of Science: 0

Resumo

The tick vector Rhipicephalus sanguineus is established as a complex of closely related species with high veterinary-medical significance, in which the presence of different genetic, morphological, and biological traits has resulted in the recognition of different lineages within taxa. One of the most striking differences in the ``temperate{''} and ``tropical{''} lineages of R. sanguineus (s.l.) is the vector competence to Ehrlichia canis, suggesting that these ticks tolerate and react differently to pathogen infection. The present study addresses the SG and MG proteome of the R. sanguineus tropical and temperate lineages and compares their proteomic profile during E. canis infection. Batches of nymphs from the two lineages were allowed to feed on naive and experimentally E. canis infected dogs and after molting, adults were dissected, and salivary glands and midgut tissues separated. Samples were screened for the presence of E. canis before proteomic analyses. The representation of the proteins identified in infected and non-infected tissues of each lineage was compared and gene ontology used for protein classification. Results highlight important differences in those proteomic profiles that added to previous reported genetic, biological, behavioral, and morphological differences, strengthening the hypothesis of the existence of two different species. Comparing infected and non-infected tissues, the results show that, while in midgut tissues the response to E. canis infection is similar in the salivary glands, the two lineages show a different pattern of protein representation. Focusing on the proteins found only in the infected condition, the data suggests that the cement cone produced during tick feeding may be implicated in pathogen infection. This study adds useful information to the debate on the controversial R. sanguineus systematic status, to the discussion related with the different vectorial competence occurring between the two lineages and identifies potential targets for efficient tick and tick-borne disease control. (AU)

• Processo FAPESP: 18/06651-7 - DESVENDANDO O CICLO DA Ehrlichia canis NO CARRAPATO Rhipicephalus sanguineus (linhagem tropical): UM PASSO ESSENCIAL PARA O CONTROLE DA ERLIQUIOSE CANINA
• Beneficiário: Maria Izabel Souza Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Regular