OOCYTES OF THE ENDANGERED GRAY CATFISH (SURUBIM-DOPARAIBA) SUBMITTED TO DIFFERENT CONSERVATION PROTOCOLS

Autor(es)

Lopes, Tais da Silva ^[1] ; Romagosa, Elizabeth ^[2]

Afiliação do(s) autor(es)

^[1] Univ Fed Espirito Santo, Dept Anim Sci, BR-29500000 Alegre, ES - Brazil

^[2] SAA, Fishery Inst, APTA, BR-05001970 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CRYOLETTERS; v. 41, n. 6, p. 323-329, NOV 2020.
• Citações Web of Science: 0

Resumo

BACKGROUND: The gray catfish known as Surubim-do-Paraiba (Steindachneridion parahybae), which is endemic to the Paraiba do Sul river basin, is on the red list of Brazilian fauna threatened with extinction and the cryopreservation of germ cells of this fish is needed in support of conservation. OBJECTIVE: We aimed to assess the effect of storage temperature on S. parahybae mature and immature oocytes. METHODS: Two trials were carried out. Trial I (TI.1-3) used 30 mature oocytes (diameter >1.8 mm) placed in cryoprotectant solutions and submitted to three different techniques. Trial II (TII.1-3) used 30 immature oocytes (diameter <1.6 mm) placed in cryoprotectant solutions and submitted to three storage temperatures (i.e., TII.1 at room temperature for 120 min; TII.2 in the freezer for 120 min; TII.3 in liquid nitrogen for 24 hours. RESULT: The mature oocytes were sensitive to every protocol used, including at room temperature. In contrast, the immature oocytes had increased sensitivity according to the temperature reduction to which they were submitted, with the treatment in liquid nitrogen causing greater damage. CONCLUSION: The immature stages exhibit more promising results, encouraging further studies using the combination of different CPSs, mainly penetrating ones, in oocyte cryopreservation protocols. (AU)

• Processo FAPESP: 17/13245-2 - Criopreservação: a formação dos cristais de gelo e sua relação com as injúrias causadas as células
• Beneficiário: Taís da Silva Lopes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 14/21215-8 - Criopreservação de ovócitos de surubim-do-Paraíba (Steindachneridion parahybae)
• Beneficiário: Taís da Silva Lopes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado