Distinct macrophage phenotypes and redox environment during the fin fold regenerative process in zebrafish

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Paredes, Lais Cavalieri ^[1] ; Luz, Rebeca Bosso dos Santos ^[1] ; Tozzi, Ollavo Nogueira ^[1] ; de Carvalho, Lucicleide angelo Silva Jungles ^[2] ; Calado, Sabrina Loise de Morais ^[2] ; Padovani, Barbara Nunes ^[3] ; Fenero, Camila Ideli Morales ^[3] ; do Amaral, Mariana Abrantes ^[3] ; de Assis, Helena Cristina da Silva ^[2] ; Camara, Niels Olsen Saraiva ^{[4, 3]} ; Braga, Tarcio Teodoro ^{[1, 5]}

Afiliação do(s) autor(es)

^[1] Univ Fed Parana, Dept Pathol, Curitiba, Parana - Brazil

^[2] Univ Fed Parana, Dept Pharmacol, Curitiba, Parana - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci 4, Dept Immunol, Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Nephrol Div, Sao Paulo - Brazil

^[5] Fiocruz Parana, Inst Carlos Chagas, Grad Program Biosci & Biotechnol, Curitiba, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Scandinavian Journal of Immunology; v. 94, n. 2 FEB 2021.
• Citações Web of Science: 1

Resumo

In contrast to mammals, zebrafish (Danio rerio) has the ability to regenerate injured sites such as different tissues present in the fin. It is known that cells of the innate immune system play essential roles in regeneration; however, some aspects of the molecular mechanisms by which these cells orchestrate regeneration remain unknown. This study aimed to evaluate the infiltration dynamics of neutrophils and macrophages in the regenerative process of fin fold in regard to the influence of the redox environment and oxidative pathways. Fin fold amputation was performed on transgenic larvae for macrophage-expressed gene 1 (mpeg1), lysozyme (lyz), myeloperoxidase (mpo) and tumour necrosis factor alpha (TNF alpha) at 3 days post-fertilization, followed by confocal microscopy imaging and measurement of the activities of oxidant and antioxidant enzymes. We observed initially an increase in the number of neutrophils (lyz:DsRed+/mpx:GFP+) and then macrophages (mpeg1+) in the injury site followed by a decrease in neutrophils at 7 days post-amputation (dpa). Moreover, macrophages switch from a pro-inflammatory to an anti-inflammatory profile throughout the process, while the activity of superoxide dismutase (SOD) increased at 1 dpa and catalase (CAT) at 5 dpa. Higher levels of lipid peroxidation were also detected during regeneration. Despite oxidative stress, there is, therefore, an antioxidant response throughout the regeneration of the caudal fin. The present work can contribute to future studies on the development of cell therapies, achieving greater effectiveness in the treatment of diseases related to the formation of fibrotic tissue. (AU)

• Processo FAPESP: 17/05264-7 - Metabolismo celular, microbiota e sistema imune: novos paradigmas na fisiopatologia das doenças renais
• Beneficiário: Niels Olsen Saraiva Câmara
• Modalidade de apoio: Auxílio à Pesquisa - Temático