Screening of 104 New Psychoactive Substances (NPS) and Other Drugs of Abuse in Oral Fluid by LC-MS-MS

Autor(es)

da Cunha, Kelly Francisco ^{[1, 2]} ; Oliveira, Karina Diniz ^{[1, 2]} ; Huestis, Marilyn A. ^[3] ; Costa, Jose Luiz ^{[2, 4]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Fac Med Sci, UNICAMP, BR-13083859 Campinas, SP - Brazil

^[2] Univ Estadual Campinas, UNICAMP, Campinas Poison Control Ctr, BR-13083859 Campinas, SP - Brazil

^[3] Thomas Jefferson Univ, Inst Emerging Hlth Profess, Philadelphia, PA 19107 - USA

^[4] Univ Estadual Campinas, Fac Pharmaceut Sci, UNICAMP, BR-13083859 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF ANALYTICAL TOXICOLOGY; v. 44, n. 7, p. 697-707, SEP 2020.
• Citações Web of Science: 3

Resumo

New psychoactive substances (NPS) are a major public health problem, primarily due to the increased number of acute poisoning cases. Detection of these substances is a challenge. The aim of this research was to develop and validate a sensitive screening method for 104 drugs of abuse, including synthetic cannabinoids, synthetic cathinones, fentanyl analogues, phenethylamines and other abused psychoactive compounds (i.e., THC, MDMA, LSD and their metabolites) in oral fluid by liquid chromatography-tandem mass spectrometry (LC-MS-MS). The Quantisal (TM) oral fluid device was used to collect oral fluid samples. The oral fluid-elution buffer mixture (500-mu L sample) was extracted with t-butyl methyl ether, and chromatographic separation was performed on a Raptor (TM) biphenyl column (100 x 2.1 mm ID, 2.7 mu m), with a total run time of 13.5 min. Limits of detection were established at three concentrations (0.05, 0.1 or 1 ng/mL) for most analytes, except for acetyl norfentanyl and mescaline (5 ng/mL). Matrix effects were generally <20% and overall extraction recoveries >60%. The highest matrix effect was observed within the synthetic cannabinoid group (PB22, -55.5%). Lower recoveries were observed for 2C-T (47.2%) and JWH-175 (58.7%). Recoveries from the Quantisal (TM) device were also evaluated for all analytes (56.7-127%), with lower recoveries noted for 25I-NBOMe, valerylfentanyl and mCPP (56.7, 63.0 and 69.9%, respectively). Drug stability in oral fluid was evaluated at 15, 60 and 90 days and at 25, 4 and -20 degrees C. As expected, greater stability was observed when samples were stored at -20 degrees C, but even when frozen, some NPS (e.g., synthetic cannabinoids) showed more than 20% degradation. The method was successfully applied to the analysis of seven authentic oral fluid samples positive for 17 different analytes. The method achieved good sensitivity and simultaneous detection of a wide range of NPS. (AU)

• Processo FAPESP: 17/02147-0 - Cromatografia líquida em uma gota e seu acoplamento com espectrometria de massas: estratégias instrumentais, desenvolvimento de materiais, automatização e aplicações analíticas
• Beneficiário: Fernando Mauro Lanças
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/11849-0 - A toxicologia das Novas Substâncias Psicoativas (NSP): epidemiologia do consumo através da análise de amostras de fluido oral e caracterização de seu metabolismo in vitro
• Beneficiário: Kelly Francisco da Cunha
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 18/00432-1 - A Toxicologia das Novas Substâncias Psicoativas (NSP): epidemiologia do consumo através da análise de amostras de cabelo e fluido oral
• Beneficiário: José Luiz da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular