A Transcriptomic Approach Provides Insights on the Mycorrhizal Symbiosis of the Mediterranean Orchid Limodorum abortivum in Nature

Autor(es)

Valadares, Rafael B. S. ^[1] ; Marroni, Fabio ^{[2, 3]} ; Sillo, Fabiano ^[4] ; Oliveira, Renato R. M. ^{[1, 5]} ; Balestrini, Raffaella ^[4] ; Perotto, Silvia ^[6]

Afiliação do(s) autor(es)

^[1] Inst Tecnol Vale, Rua Boaventura da Silva 955, BR-66050000 Belem, Para - Brazil

^[2] Univ Udine, Dipartimento Sci Agroalimentari Ambientali & Anim, Via Sci, I-33100 Udine - Italy

^[3] Ist Genom Applicata, Via Linussio 51, I-33100 Udine - Italy

^[4] CNR, Ist Protez Sostenibile Piante, Viale PA Mattioli 25, I-10125 Turin - Italy

^[5] Univ Fed Minas Gerais, Inst Ciencias Biol, Av Pres Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil

^[6] Univ Torino, Dipartimento Sci Vita & Biol Sistemi, Viale Mattioli 25, I-10125 Turin - Italy

• Tipo de documento: Artigo Científico
• Fonte: PLANTS-BASEL; v. 10, n. 2 FEB 2021.
• Citações Web of Science: 0

Resumo

The study of orchid mycorrhizal interactions is particularly complex because of the peculiar life cycle of these plants and their diverse trophic strategies. Here, transcriptomics has been applied to investigate gene expression in the mycorrhizal roots of Limodorum abortivum, a terrestrial mixotrophic orchid that associates with ectomycorrhizal fungi in the genus Russula. Our results provide new insights into the mechanisms underlying plant-fungus interactions in adult orchids in nature and in particular into the plant responses to the mycorrhizal symbiont(s) in the roots of mixotrophic orchids. Our results indicate that amino acids may represent the main nitrogen source in mycorrhizal roots of L. abortivum, as already suggested for orchid protocorms and other orchid species. The upregulation, in mycorrhizal L. abortivum roots, of some symbiotic molecular marker genes identified in mycorrhizal roots from other orchids as well as in arbuscular mycorrhiza, may mirror a common core of plant genes involved in endomycorrhizal symbioses. Further efforts will be required to understand whether the specificities of orchid mycorrhiza depend on fine-tuned regulation of these common components, or whether specific additional genes are involved. (AU)

• Processo FAPESP: 12/50266-4 - Identificacao e caracterizacao de genes e proteinas envolvidas na regulacao do desenvolvimento de micorrizas em orquideas.
• Beneficiário: Fernando Dini Andreote
• Modalidade de apoio: Auxílio à Pesquisa - Regular