| Texto completo | |
| Autor(es): Mostrar menos - |
Ortega, Francisco G.
[1]
;
Regiart, Matias D.
[2]
;
Rodriguez-Martinez, Alba
[3, 4]
;
de Miguel-Perez, Diego
[3]
;
Serrano, Maria J.
[3]
;
Lorente, Jose A.
[3, 4]
;
Tortella, Gonzalo
[5, 6]
;
Rubilar, Olga
[5, 6]
;
Sapag, Karim
[7]
;
Bertotti, Mauro
[2]
;
Fernandez-Baldo, Martin A.
[8]
Número total de Autores: 11
|
| Afiliação do(s) autor(es): | [1] Balearic Isl Hlth Res Inst IdISBa, Palma De Mallorca 07010 - Spain
[2] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, LSEME, Lab Sensores Eletroquim & Metodos Eletroanalit, BR-05513970 Sao Paulo, SP - Brazil
[3] Univ Granada, Ctr Genom & Oncol Res Pfizer, Liquid Biopsy & Metastasis Res Grp, GENYO, Granada 18016 - Spain
[4] Univ Granada, Fac Med PTS, Legal Med & Toxicol Dept, Lab Genet Identificat, Granada 18016 - Spain
[5] Univ La Frontera, Dept Ingn Quim, Temuco 4811230 - Chile
[6] Ctr Excelencia Invest Biotecnol Aplicada Medio Am, Temuco 4811230 - Chile
[7] Univ Nacl San Luis, Lab Solidos Porosos, INFAP, San Luis - Argentina
[8] Univ Nacl San Luis, Dept Quim, INQUISAL, D5700BWS, San Luis - Argentina
Número total de Afiliações: 8
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Analytical Chemistry; v. 93, n. 2, p. 1143-1153, JAN 19 2021. |
| Citações Web of Science: | 0 |
| Resumo | |
This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL(-1), with a wide linear range of 2 to 1000 pg mL(-)(1), while for CD81, the limit of detection was 3 pg mL(-1), with a wide linear range of 0.01 to 10 ng mL(-1). Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis. (AU) | |
| Processo FAPESP: | 18/08782-1 - Sensores químicos miniaturizados e integrados: novas plataformas de fabricação para aplicações biológicas, clínicas e ambientais |
| Beneficiário: | Mauro Bertotti |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 19/06293-6 - Investigações em sistemas biológicos empregando sensores eletroquímicos miniaturizados |
| Beneficiário: | Daniel Matias Gaston Regiart |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |