Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients

Author(s):Show less -

Ortega, Francisco G. ^[1] ; Regiart, Matias D. ^[2] ; Rodriguez-Martinez, Alba ^{[3, 4]} ; de Miguel-Perez, Diego ^[3] ; Serrano, Maria J. ^[3] ; Lorente, Jose A. ^{[3, 4]} ; Tortella, Gonzalo ^{[5, 6]} ; Rubilar, Olga ^{[5, 6]} ; Sapag, Karim ^[7] ; Bertotti, Mauro ^[2] ; Fernandez-Baldo, Martin A. ^[8]

Affiliation

^[1] Balearic Isl Hlth Res Inst IdISBa, Palma De Mallorca 07010 - Spain

^[2] Univ Sao Paulo, Dept Fundamental Chem, Inst Chem, LSEME, Lab Sensores Eletroquim & Metodos Eletroanalit, BR-05513970 Sao Paulo, SP - Brazil

^[3] Univ Granada, Ctr Genom & Oncol Res Pfizer, Liquid Biopsy & Metastasis Res Grp, GENYO, Granada 18016 - Spain

^[4] Univ Granada, Fac Med PTS, Legal Med & Toxicol Dept, Lab Genet Identificat, Granada 18016 - Spain

^[5] Univ La Frontera, Dept Ingn Quim, Temuco 4811230 - Chile

^[6] Ctr Excelencia Invest Biotecnol Aplicada Medio Am, Temuco 4811230 - Chile

^[7] Univ Nacl San Luis, Lab Solidos Porosos, INFAP, San Luis - Argentina

^[8] Univ Nacl San Luis, Dept Quim, INQUISAL, D5700BWS, San Luis - Argentina

• Document type: Journal article
• Source: Analytical Chemistry; v. 93, n. 2, p. 1143-1153, JAN 19 2021.
• Web of Science Citations: 0

Abstract

This study is focused on identifying novel epithelial markers in circulating extracellular vesicles (EVs) through the development of a dual sandwich-type electrochemical paper-based immunosensor for Claudin 7 and CD81 determination, as well as its validation in breast cancer (BC) patients. This immunosensor allows for rapid, sensitive, and label-free detection of these two relevant BC biomarkers. Under optimum conditions, the limit of detection for Claudin 7 was 0.4 pg mL(-1), with a wide linear range of 2 to 1000 pg mL(-)(1), while for CD81, the limit of detection was 3 pg mL(-1), with a wide linear range of 0.01 to 10 ng mL(-1). Finally, we validated Claudin 7 and CD81 determination in EVs from 60 BC patients and 20 healthy volunteers, reporting higher diagnostic accuracy than the one observed with classical diagnostic markers. This analysis provides a low-cost, specific, versatile, and user-friendly strategy as a robust and reliable tool for early BC diagnosis. (AU)

• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/06293-6 - Investigations in biological systems by using miniaturized electrochemical sensors
• Grantee: Daniel Matias Gaston Regiart
• Support Opportunities: Scholarships in Brazil - Post-Doctoral