Investigations in biological systems by using miniaturized electrochemical sensors

• Grant number: 19/06293-6
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: July 01, 2019
• End date: June 30, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Mauro Bertotti
• Grantee: Daniel Matias Gaston Regiart
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications, AP.TEM
• Associated scholarship(s): 20/11722-0 - Development of biosensors based on microelectrodes for in vitro and in vivo monitoring of neurometabolic markers in the rats brain, BE.EP.PD

Abstract

The main goal of the research project is based on the development of microelectrodes and microelectrodes arrays for further use in biological systems, particularly in the monitoring of neurotransmitters release, as well as in the study of metabolic processes. Experiments will be performed using devices mounted in different configurations, depending on the size of the cells and the desired information about the cellular system. In order to improve the sensitivity and selectively of the fabricated sensors response, strategies based on the modification of the electrode surface will be employed, if necessary. The project will be carried out in collaboration with researchers from the Department of Biochemistry, hence we envisage a multidisciplinary approach with emphasis on excellence and highest scientific standards. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

JOFRE, CLAUDIO F.; REGIART, MATIAS; FERNANDEZ-BALDO, MARTIN A.; BERTOTTI, MAURO; RABA, JULIO; MESSINA, GERMAN A.. Electrochemical microfluidic immunosensor based on TES-AuNPs@Fe3O4 and CMK-8 for IgG anti-Toxocara canis determination. Analytica Chimica Acta, v. 1096, p. 120-129, FEB 1 2020. (19/06293-6)

ORTEGA, FRANCISCO G.; REGIART, MATIAS D.; RODRIGUEZ-MARTINEZ, ALBA; DE MIGUEL-PEREZ, DIEGO; SERRANO, MARIA J.; LORENTE, JOSE A.; TORTELLA, GONZALO; RUBILAR, OLGA; SAPAG, KARIM; BERTOTTI, MAURO; et al. Sandwich-Type Electrochemical Paper-Based Immunosensor for Claudin 7 and CD81 Dual Determination on Extracellular Vesicles from Breast Cancer Patients. Analytical Chemistry, v. 93, n. 2, p. 1143-1153, JAN 19 2021. (18/08782-1, 19/06293-6)

REGIART, MATIAS; KUMAR, ABHISHEK; GONCALVES, JOSUE M.; SILVA JUNIOR, GILBERTO J.; MASINI, JORGE CESAR; ANGNES, LUCIO; BERTOTTI, MAURO. An Electrochemically Synthesized Nanoporous Copper Microsensor for Highly Sensitive and Selective Determination of Glyphosate. CHEMELECTROCHEM, v. 7, n. 7, p. 1558-1566, APR 1 2020. (18/16896-7, 19/14418-3, 18/08782-1, 19/06293-6)

JOFRE, CLAUDIO F.; REGIART, MATIAS; FERNANDEZ-BALDO, MARTIN A.; BERTOTTI, MAURO; RABA, JULIO; MESSINA, GERMAN A.. Electrochemical microfluidic immunosensor based on TES-AuNPs@Fe₃O₄ and CMK-8 for IgG anti-Toxocara canis determination. Analytica Chimica Acta, v. 1096, p. 10-pg., 2020-02-01. (19/06293-6)

PIGUILLEM, SOFIA V.; REGIART, MATIAS; BERTOTTI, MAURO; RABA, JULIO; MESSINA, GERMAN A.; FERNANDEZ-BALDO, MARTIN A.. Microfluidic fluorescence immunosensor using ZnONFs for invasive aspergillosis determination. Microchemical Journal, v. 159, DEC 2020. (19/06293-6, 18/08782-1)

DIP GANDARILLA, ARIAMNA MARIA; REGIART, MATIAS; BERTOTTI, MAURO; CORREA GLORIA, JULIANE; MORAIS MARIUBA, LUIS ANDRE; BRITO, WALTER RICARDO. One-step enzyme-free dual electrochemical immunosensor for histidine-rich protein 2 determination. RSC ADVANCES, v. 11, n. 1, p. 408-415, JAN 1 2021. (18/08782-1, 19/06293-6)

REGIART, MATIAS; GIMENEZ, ALBA MARINA; LOPES, ALEXANDRE T.; CARRENO, MARCELO N. P.; BERTOTTI, MAURO. Ultrasensitive microfluidic electrochemical immunosensor based on electrodeposited nanoporous gold for SOX-2 determination. Analytica Chimica Acta, v. 1127, p. 122-130, AUG 29 2020. (18/08782-1, 19/06293-6)

REGIART, MATIAS; GIMENEZ, ALBA MARINA; MARQUES, RODOLFO F.; SOARES, IRENE S.; BERTOTTI, MAURO. Microfluidic device based on electrodeposited Nanoporous Gold/Carbon Nanotubes for Plasmodium vivax detection. SENSORS AND ACTUATORS B-CHEMICAL, v. 340, AUG 1 2021. (16/50108-0, 19/06293-6, 18/08782-1)

SILVA JUNIOR, GILBERTO J.; GUIMARAES SELVA, JESSICA SOARES; SUKERI, ANANDHAKUMAR; GONCALVES, JOSUE M.; REGIART, MATIAS; BERTOTTI, MAURO. Fabrication of dendritic nanoporous gold via a two-step amperometric approach: Application for electrochemical detection of methyl parathion in river water samples. Talanta, v. 226, MAY 2021. (19/14418-3, 18/16896-7, 18/08782-1, 19/06293-6)