Miniaturized large-scale devices for in-situ analysis: fabrication, characterization and applications

• Grant number: 23/00246-1
• Support Opportunities: Research Projects - Thematic Grants
• Start date: May 01, 2024
• End date: April 30, 2029
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Mauro Bertotti
• Grantee: Mauro Bertotti
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Pesquisadores principais: Thiago Regis Longo Cesar da Paixão
• Associated researchers: Renato Sousa Lima ; William Reis de Araujo
• Associated research grant(s): 24/06789-0 - Multi-user Equipment approved in grant 23/00246-1: SPELECRAMAN, AP.EMU
• Associated scholarship(s): 24/06773-6 - A FACILE LABEL-FREE ELECTROCHEMICAL APTASENSOR FOR THE DETECTION OF MARINE BIOLOGICAL TOXINS BASED ON NANOPOROUS GOLD-MODIFIED ELECTRODE, BP.PD; 25/00748-2 - Monitoring of the NO release in rat brains with miniaturized electrochemical sensors, BP.DD; 24/18077-4 - Fabrication of electrochemical nanosensors for biological applications using SECM, BP.PD; + associated scholarships 24/10870-7 - Ultradense electrochemical chip, microfluidics and machine learning to monitoring cell viability: a promising platform of cancer drug screening., BP.DD; 24/15626-7 - Quantification of Levofloxacin (LEV) on the electrode fabricated using a 3D pen, BP.TT; 24/09761-9 - New platforms for the fabrication of electrochemical sensors towards ethanol detection, BP.IC; 24/07124-1 - Nanoelectrodes fabrication for uses with Scanning Electrochemical Microscopy, BP.TT - associated scholarships

Abstract

The research project focuses on the fabrication and implementation of integrated miniaturized systems for the online monitoring of chemical species of environmental, biological, industrial, and clinical interest. The proposed devices will consist of miniaturized electrochemical, physical, and optical sensors, and emphasis will be given to constructing prototypes with high robustness and compatibility for large-scale production, so they can be used in routine analysis. The selectivity and sensitivity of the sensors concerning the target molecules depend on the nature of the samples, and enhanced performance can be achieved with platforms designed using new materials and nanomaterials. Sensors that operate under real conditions, for a long time, and without loss of response are desired. In special cases, the coupling of these devices to microfluidic systems will consist of a relevant strategy to enable the detection of more complex samples with greater precision and analytical frequency. The main aspects are: i. miniaturized sensors for obtaining in situ and real-time chemical information on systems of biological interest; ii. combination of paper substrates and other materials with microfluidic structures; iii. Sensors built on flexible platforms compatible with monitoring directly on the human body and plant leaves (wearable devices) for health and precision agriculture applications, respectively; iv. disposable sensors manufactured on a large scale for environmental, industrial, and food applications; and v. new accessible and scalable clinical diagnostic tools. In the presented scenario, the project is fully aligned with some of the UN Sustainable Development Goals (SDGs). (AU)

Scientific publications

(17)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FUKANA, NUTNAREE; PARK, JOOWON; JUNIOR, GILBERTO J. SILVA; MALSICK, LAUREN E.; GALLICHOTTE, EMILY N.; EBEL, GREGORY D.; GEISS, BRIAN J.; DANDY, DAVID S.; BERTOTTI, MAURO; NACAPRICHA, DUANGJAI; et al. Magnetophoretic slider assay for electrochemical detection of SARS-cov-2 nucleocapsid protein in nasal swab samples. BIOSENSORS & BIOELECTRONICS, v. 271, p. 9-pg., 2024-12-12. (22/09861-8, 23/00246-1)

BERASARTE, IRATI; ALBIZU, GORKA; SANTOS, WENDEL FERREIRA; DE LIMA, LUCAS FELIPE; OSTRA, MIREN; VIDAL, MAIDER; DE ARAUJO, WILLIAM REIS. Chemometrics and digital image colorimetry approaches applied to paper-based analytical devices: A review. Analytica Chimica Acta, v. 1339, p. 19-pg., 2025-01-18. (18/08782-1, 23/00246-1, 22/03250-7)

CARVALHO, MAYANE S.; ROCHA, RAQUEL G.; NASCIMENTO, AMANDA BEATRIZ; ARAUJO, DIELE A. G.; PAIXAO, THIAGO R. L. C.; LOPES, OSMANDO F.; RICHTER, EDUARDO M.; MUNOZ, RODRIGO A. A.. Enhanced electrochemical performance of 3D-printed electrodes via blue-laser irradiation and (electro)chemical treatment. Electrochimica Acta, v. 506, p. 12-pg., 2024-09-10. (22/01810-5, 23/00246-1)

CAMARGO, JESSICA ROCHA; ORZARI, LUIZ OTAVIO; RODRIGUES, JESSICA DE SOUZA; DE LIMA, LUCAS FELIPE; PAIXAO, THIAGO REGIS LONGO CESAR; FRACETO, LEONARDO FERNANDES; JANEGITZ, BRUNO CAMPOS. Advancements in disposable electrochemical systems for sustainable agriculture monitoring: Trends, gaps, and applied examples. TRAC-TRENDS IN ANALYTICAL CHEMISTRY, v. 180, p. 14-pg., 2024-09-13. (17/21004-5, 22/03399-0, 21/10639-5, 19/04758-1, 19/23342-0, 23/12589-0, 23/00246-1, 23/06793-4, 19/23177-0, 22/00509-0, 23/00335-4)

BOANE, ANASTACIO A.; ARAUJO, DIELE A. G.; MUNOZ, RODRIGO A. A.; PAIXAO, THIAGO R. L. C.; RICHTER, EDUARDO M.. Sulfamethoxazole Detection in Milk Using a Carbon-Based Printed Electrode: Enhanced Selectivity and Response Stability by Pulse-Amperometry. Journal of the Electrochemical Society, v. 172, n. 2, p. 8-pg., 2025-02-01. (22/01810-5, 23/00246-1, 18/08782-1)

LIMA, CAMILA DIANA; ARANTES, LUCIANO C.; MACHADO, LARA L.; PAIXAO, THIAGO R. L. C.; DOS SANTOS, WALLANS T. P.. Synthetic Cathinones' Comprehensive Screening and Classification by Voltammetric and Chemometric Analyses: A Powerful Method for On-Site Forensic Applications. Analytical Chemistry, v. 96, n. 44, p. 8-pg., 2024-10-25. (23/00246-1)

SEVENE, DANIEL R.; MATIAS, TIAGO A.; ARAUJO, DIELE A. G.; INOQUE, NELIO I. G.; NAKAMURA, MARCELO; PAIXAO, THIAGO R. L. C.; MUNOZ, RODRIGO A. A.. Laser-Induced Graphene for Electrochemical Sensing of Antioxidants in Biodiesel. ACS OMEGA, v. N/A, p. 10-pg., 2024-11-19. (22/01810-5, 23/00246-1)

FERREIRA, BRUNO; ARANTES, IANA V. S.; GONGONI, JULIANA L. M.; PRADELA-FILHO, LAURO A.; PAIXAO, THIAGO R. L. C.. Stencil-printed graphene electrodes for affordable electrochemical sensing of capsaicin. Microchemical Journal, v. 207, p. 7-pg., 2024-12-01. (19/15065-7, 18/08782-1, 23/00246-1, 21/00205-8)

BOTTELLI, ELISA D.; DE LIMA, LUCAS F.; PAIXAO, THIAGO R. L. C.; DE ARAUJO, WILLIAM R.. Laser-scribed graphene toward scalable fabrication of electrochemical paper-based devices for lidocaine detection in forensic and pharmaceutical samples. Electrochimica Acta, v. 507, p. 10-pg., 2024-10-07. (23/12589-0, 18/08782-1, 23/00246-1, 22/03250-7)

DORETTO, DANIEL S.; CORSATO, PAULA C. R.; SILVA, CHRISTIAN O.; PESSOA, JAMES C.; VIEIRA, LUIS C. S.; DE ARAUJO, WILLIAM R.; SHIMIZU, FLAVIO M.; PIAZZETTA, MARIA H. O.; GOBBI, ANGELO L.; RIBEIRO, IRIS R. S.; et al. Ultradense Electrochemical Chip and Machine Learning for High-Throughput, Accurate Anticancer Drug Screening. ACS SENSORS, v. N/A, p. 12-pg., 2024-11-29. (23/00246-1, 24/10870-7)

VELOSO, WILLIAM B.; PAIXAO, THIAGO R. L. C.; MELONI, GABRIEL N.. The Current Shortcomings and Future Possibilities of 3D Printed Electrodes. Analytical Chemistry, v. 96, n. 36, p. 5-pg., 2024-08-28. (22/03382-0, 18/08782-1, 23/00246-1, 21/00800-3)

DE LIMA, LUCAS FELIPE; FERREIRA, ANDRE LOPES; DO NASCIMENTO, GUIDA HELLEN MOTA; CARDOSO, LUANA PEREIRA; DE JESUS, MARCELO BISPO; DE ARAUJO, WILLIAM REIS. Tongue depressor (bio)sensors: A fast decentralized self-testing of salivary biomarkers for personalized medicine. CHEMICAL ENGINEERING JOURNAL, v. 494, p. 13-pg., 2024-06-18. (22/09892-0, 23/03483-4, 23/05204-5, 23/12589-0, 23/06143-0, 23/00246-1, 18/08782-1, 22/03250-7)

VELOSO, WILLIAM B.; MELONI, GABRIEL N.; ARANTES, IANA V. S.; PRADELA-FILHO, LAURO A.; MUNOZ, RODRIGO A. A.; PAIXAO, THIAGO R. L. C.. Gold film deposition by infrared laser photothermal treatment on 3D-printed electrodes: electrochemical performance enhancement and application. ANALYST, v. 149, n. 15, p. 11-pg., 2024-06-17. (21/00205-8, 22/03382-0, 21/00800-3, 19/15065-7, 23/00246-1, 22/11346-4)

OLIVEIRA, ANA CLARA M.; RICHTER, EDUARDO M.; PAIXAO, THIAGO R. L. C.; ARAUJO, DIELE A. G.; MUNOZ, RODRIGO A. A.. Electrochemical determination of nitazoxanide in environmental and pharmaceutical samples using a boron-doped diamond electrode. Microchemical Journal, v. 207, p. 8-pg., 2024-11-15. (23/00246-1)

SARAIVA, DOUGLAS P. M.; FERREIRA, BRUNO; RIBEIRO, LEONARDO M. A.; PAIXAO, THIAGO R. L. C.; BERTOTTI, MAURO. Cost-effective quantification of uric acid using niobium oxide and graphene oxide-modified pencil-drawn electrodes on PVC substrates. ANALYTICAL METHODS, v. 16, n. 43, p. 6-pg., 2024-09-30. (23/00246-1, 23/02572-3)

AYRES, LUCAS B.; PIMENTEL, GABRIEL J. C.; COSTA, JULIANA N. Y.; PIAZZETTA, MARIA H. O.; GOBBI, ANGELO L.; SHIMIZU, FLAVIO M.; GARCIA, CARLOS D.; LIMA, RENATO S.. Ultradense Array of On-Chip Sensors for High-Throughput Electrochemical Analyses. ACS SENSORS, v. 9, n. 8, p. 9-pg., 2024-07-12. (22/04397-1, 23/02080-3, 23/00246-1)

FERREIRA, BRUNO; ARANTES, IANA V. S.; CRAPNELL, ROBERT D.; BERNALTE, ELENA; BANKS, CRAIG E.; PAIXAO, THIAGO R. L. C.. 3D printing pen for patterning electrochemical sensors on a paper platform for capsaicin detection. ANALYST, v. N/A, p. 8-pg., 2025-01-16. (19/15065-7, 23/00246-1)