Miniaturized and/or microfluidic analytical devices for biological applications

• Grant number: 21/00205-8
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: October 01, 2021
• End date: September 30, 2025
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Thiago Regis Longo Cesar da Paixão
• Grantee: Lauro Antonio Pradela Filho
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications, AP.TEM
• Associated scholarship(s): 22/11346-4 - 3D-printed microfluidic systems for biological analytical applications, BE.EP.PD

Abstract

The development of new devices for fast decision-making is of great interest in the area of Analytical Chemistry since the analyzes performed in laboratories can take longer for a result when considered the sampling, transport, and analysis of the samples. Thus, the research has been directed to developing new devices that could rapidly and efficiently work at the sampling site, reducing the errors and contamination risks associated with the manual acquisition of samples, storage, and transport. These devices are usually fabricated on microfluidic and/or miniaturized platforms, ensuring better portability to the systems. Also, these devices offer fast analysis and require low sample volumes. As a result, this work consists of developing integrated strategies to monitor species such as cortisol, lactate, nitrite, and glucose in saliva samples since they are important biomarkers for disease diagnoses. Electrochemical and optical detectors will be used to produce portable devices. The devices will be integrated with steps of sample pre-treatment, chemical reactions, separation, and detection. Sensors operating in real conditions for long-term use without loss of response will be desired to determine the species with biological relevance. The objective consists of developing new devices that satisfy the analytical requirements, such as fast response, resistance to the fouling effects, high selectivity, accuracy, and precision. Besides, fabrication methods must meet technological demands, such as large-scale production with low-cost, low energy consumption, and robust electronics. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FERREIRA, BRUNO; ARANTES, IANA V. S.; SARAIVA, DOUGLAS P. M.; PRADELA-FILHO, LAURO A.; BERTOTTI, MAURO; PAIXAO, THIAGO R. L. C.. Commercial ink-coated PVC: No longer abrading conventional PVC surfaces for electrode fabrication using pencil drawing. Microchemical Journal, v. 198, p. 7-pg., 2024-02-15. (23/02572-3, 21/00205-8, 19/15065-7, 18/08782-1, 22/11346-4)

FERREIRA, BRUNO; ARANTES, IANA V. S.; GONGONI, JULIANA L. M.; PRADELA-FILHO, LAURO A.; PAIXAO, THIAGO R. L. C.. Stencil-printed graphene electrodes for affordable electrochemical sensing of capsaicin. Microchemical Journal, v. 207, p. 7-pg., 2024-12-01. (19/15065-7, 18/08782-1, 23/00246-1, 21/00205-8)

PRADELA-FILHO, LAURO A. A.; VELOSO, WILLIAM B. B.; MEDEIROS, DEBORA N.; LINS, RENATO S. O.; FERREIRA, BRUNO; BERTOTTI, MAURO; PAIXAO, THIAGO R. L. C.. Patterning (Electro)chemical Treatment-Free Electrodes with a 3D Printing Pen. Analytical Chemistry, v. 95, n. 28, p. 10-pg., 2023-06-22. (21/00205-8, 19/00811-5, 18/08782-1)

GONGONI, JULIANA L. M.; FILHO, LAURO A. P.; DE FARIAS, DAVI M.; ARANTES, IANA V. S.; PAIXAO, THIAGO R. L. C.. Modulating the Electrochemical Response of Eco-Friendly Laser-Pyrolyzed Paper Sensors Applied to Nitrite Determination. CHEMELECTROCHEM, v. 10, n. 1, p. 8-pg., 2022-12-21. (19/15065-7, 21/00205-8, 19/00811-5)

PRADELA-FILHO, LAURO A.; GONGONI, JULIANA L. M.; ARANTES, IANA V. S.; DE FARIAS, DAVI M.; PAIXAO, THIAGO R. L. C.. Controlling the Inkjet Printing Process for Electrochemical (Bio)Sensors. ADVANCED MATERIALS TECHNOLOGIES, v. N/A, p. 9-pg., 2023-01-29. (19/15065-7, 21/00205-8, 19/00811-5, 18/08782-1)

DE FARIAS, DAVI M.; PRADELA-FILHO, LAURO A.; ARANTES, IANA V. S.; GONGONI, JULIANA L. M.; VELOSO, WILLIAM B.; MELONI, GABRIEL N.; PAIXAO, THIAGO R. L. C.. Sulfanilamide Electrochemical Sensor Using Phenolic Substrates and CO₂ Laser Pyrolysis. ACS APPLIED MATERIALS & INTERFACES, v. 15, n. 48, p. 9-pg., 2023-11-20. (19/15065-7, 18/08782-1, 21/00205-8)

VELOSO, WILLIAM B.; MELONI, GABRIEL N.; ARANTES, IANA V. S.; PRADELA-FILHO, LAURO A.; MUNOZ, RODRIGO A. A.; PAIXAO, THIAGO R. L. C.. Gold film deposition by infrared laser photothermal treatment on 3D-printed electrodes: electrochemical performance enhancement and application. ANALYST, v. 149, n. 15, p. 11-pg., 2024-06-17. (21/00205-8, 22/03382-0, 21/00800-3, 19/15065-7, 23/00246-1, 22/11346-4)

ATAIDE, VANESSA N.; PRADELA-FILHO, LAURO A.; AMEKU, WILSON A.; NEGAHDARY, MASOUD; OLIVEIRA, THAWAN G.; SANTOS, BERLANE G.; PAIXAO, THIAGO R. L. C.; ANGNES, LUCIO. Paper-based electrochemical biosensors for the diagnosis of viral diseases. Microchimica Acta, v. 190, n. 7, p. 17-pg., 2023-07-01. (19/22126-2, 18/16896-7, 18/08782-1, 14/50867-3, 21/00205-8, 18/14462-0, 17/13137-5)

PRADELA-FILHO, LAURO A.; VELOSO, WILLIAM B.; ARANTES, IANA V. S.; GONGONI, JULIANA L. M.; DE FARIAS, DAVI M.; ARAUJO, DIELE A. G.; PAIXAO, THIAGO R. L. C.. Paper-based analytical devices for point-of-need applications. Microchimica Acta, v. 190, n. 5, p. 23-pg., 2023-05-01. (21/00205-8, 19/15065-7, 22/11346-4)