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Use of disposable electrodes on the development of biosensors for cancer biomarkers detection

Grant number: 12/24079-2
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): October 01, 2013
Effective date (End): August 20, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Ronaldo Censi Faria
Grantee:Carolina Venturini Uliana
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The determination of different tumor biomarkers may aid in early diagnosis of disease and the identification of the cause, leading to a personalized treatment of the patient. Tumor biomarkers are substances present in tumor, blood or other fluids, as urine and saliva, produced primarily by the tumor itself or secondarily by other organs as a response to the presence of tumor. One possibility for the detection of biomarkers present in the serum involves the use of biosensors and it presents numerous advantages, such as high specificity of affinity reactions, speed, requiring small sample volumes, among others. The objective of this proposal is the development of disposable biosensors for determining cancer biomarkers. During postdoctoral, it is intended the development of immunosensors and / or genosensors through immobilization of specific antibodies and DNA probes for protein biomarkers related to breast cancer, such as estrogen receptors (ER) and HER2. The detection will be performed using specific labeled antibodies that will interact with the biomarkers and provide an electrochemical response on the electrodes. If the determination of both biomarkers with low detection limits have success, the method may help in the early breast cancer diagnosis and also for monitoring patients hospitalized, in a quickly and inexpensively manner.

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ULIANA, CAROLINA V.; PEVERARI, CAMILA R.; AFONSO, ANDRE S.; COMINETTI, MARCIA R.; FARIA, RONALDO C. Fully disposable microfluidic electrochemical device for detection of estrogen receptor alpha breast cancer biomarker. BIOSENSORS & BIOELECTRONICS, v. 99, p. 156-162, JAN 15 2018. Web of Science Citations: 28.
AFONSO, ANDRE S.; ULIANA, CAROLINA V.; MARTUCCI, DIEGO H.; FARIA, RONALDO C. Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications. Talanta, v. 146, p. 381-387, JAN 1 2016. Web of Science Citations: 24.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.

Filed patent(s) as a result of this research project

DISPOSITIVO MICROFLUÍDICO E MÉTODO DE FABRICAÇÃO DE DISPOSITIVO MICROFLUÍDICO BR1020160109841 - Universidade Federal de São Carlos (UFSCAR) . Ronaldo Censi Faria; Carolina Venturini Uliana; André Santiago Afonso - May 2016, 13

DISPOSITIVO MICROFLUÍDICO E MÉTODO DE FABRICAÇÃO DE DISPOSITIVO MICROFLUÍDICO PCT/BR2017/000052 - Universidade Estadual de Campinas (UNICAMP) . Ronaldo Censi Faria; Carolina Venturini Uliana; André Santiago Afonso - May 2017, 15