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Development of immunosensor using disposable carbon electrodes for determination of cancer biomarkers and pathogenic bacteria


This project proposes the development of immunosensor for determination of biomarker for cancer diagnosis and pathogenic bacteria in food using disposable carbon electrode. The sensor devices will be developed immobilizing specific antibodies for the analytes using layer-by-layer techniques with gold nanoparticle and polyions films over the disposable carbon electrodes. Magnetic particles densely modified with peroxidase and secondary antibody will be used for capture, pre-concentration and detection of the analytes. For the analytes' determination techniques of amperometry and voltammetry as well as electrochemiluminescence using light-emitting compounds like ruthenium complex will be evaluated. Disposable carbon electrodes will be constructed and the immunosensors will be evaluated using a microfluidic system that will allow the analytes determination of with low detection limit, high selectivity and low cost. Electrodes characterization will be done using spectroscopic techniques and microscopic as MEV and AFM among others and using electrochemical impedance. Finally, we intend to detect the analytes in different spiked samples as well as we will try to get real samples of contaminated food and serum samples that contain the biomarkers. For cancer biomarkers we intend to evaluate the multiplexed determination of three or more biomarkers with the objective to achieve more accurate diagnosis. The project has also the objective to produce papers in high-impact journals and primarily contribute to formation of high quality human resources for research. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
AFONSO, ANDRE S.; ULIANA, CAROLINA V.; MARTUCCI, DIEGO H.; FARIA, RONALDO C.. Simple and rapid fabrication of disposable carbon-based electrochemical cells using an electronic craft cutter for sensor and biosensor applications. Talanta, v. 146, p. 381-387, . (13/06750-1, 12/24079-2, 12/09752-2)
ULIANA, CAROLINA V.; PEVERARI, CAMILA R.; AFONSO, ANDRE S.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Fully disposable microfluidic electrochemical device for detection of estrogen receptor alpha breast cancer biomarker. BIOSENSORS & BIOELECTRONICS, v. 99, p. 156-162, . (13/06750-1, 12/24079-2, 15/19890-1)

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Filed patent(s) as a result of this research project

DISPOSITIVO MICROFLUÍDICO E MÉTODO DE FABRICAÇÃO DE DISPOSITIVO MICROFLUÍDICO BR1020160109841 - Universidade Federal de São Carlos (UFSCAR) . Ronaldo Censi Faria; Carolina Venturini Uliana; André Santiago Afonso - May 2016, 13

DISPOSITIVO MICROFLUÍDICO E MÉTODO DE FABRICAÇÃO DE DISPOSITIVO MICROFLUÍDICO PCT/BR2017/000052 - Universidade Estadual de Campinas (UNICAMP) . Ronaldo Censi Faria; Carolina Venturini Uliana; André Santiago Afonso - May 2017, 15