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Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases

Grant number: 17/24053-7
Support type:Regular Research Grants
Duration: June 01, 2018 - May 31, 2020
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Ronaldo Censi Faria
Grantee:Ronaldo Censi Faria
Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The development of simple, fast, and low-cost methods for protein biomarkers detection for disease diagnosis has been the focuses of our research Group in the last years. For the next period, the aim is continuing the research in this area with focus on the development of new devices and methods to improve the scientific knowledge in the field and advances in technological innovation. In this new step, we intend to work in the microRNA detection for cancer diagnosis, what is a great challenge since we do not have previous experience with this type of analyte. This project will be carried out in partnership with Hospital do Câncer de Barretos, which we have a scientific and technical cooperation accord and a project approved in ethical committees of both institutions. In the field of biomarkers detection, we have different works focusing in the protein detection for diagnosis of diseases as cancer and Alzheimer. In this project, we will work in the development of low-cost and disposable electrochemical methods for applications with and without microfluidics. In this way, biosensors will be constructed by nanostructure film formed over the disposable electrodes following by covalent immobilization of antibodies for the protein biomarkers or DNA or RNA probes complementary to the targets microRNA. Magnetic particles havely decorated with antibodies or DNA or RNA probes will be used for capture, separation, preconcentration and detection of the analytes. The application of the analytical devices will be evaluated in patient and crop samples that will be provided by the partner research groups. The project has also the aim to form human resources in the different levels and the publication of the papers in national and international journals as well as the deposition of patents. (AU)

Scientific publications (6)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FAVA, ELSON LUIZ; DO PRADO, THIAGO MARTIMIANO; GARCIA-FILHO, AMAURI; SILVA, TIAGO ALMEIDA; CINCOTTO, FERNANDO HENRIQUE; DE MORAES, FERNANDO CRUZ; FARIA, RONALDO CENSI; FATIBELLO-FILHO, ORLANDO. Non-enzymatic electrochemical determination of creatinine using a novel screen-printed microcell. Talanta, v. 207, JAN 15 2020. Web of Science Citations: 0.
FAVA, ELSON LUIZ; DO PRADO, THIAGO MARTIMIANO; SILVA, TIAGO ALMEIDA; DE MORAES, FERNANDO CRUZ; FARIA, RONALDO CENSI; FATIBELLO-FILHO, ORLANDO. New Disposable Electrochemical Paper-based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample. Electroanalysis, JAN 2020. Web of Science Citations: 0.
FREITAS, TAYANE A.; PROENCA, CAMILA A.; BALDO, THAISA A.; MATERON, ELSA M.; WONG, ADEMAR; MAGNANI, RODRIGO F.; FARIA, RONALDO C. Ultrasensitive immunoassay for detection of Citrus tristeza virus in citrus sample using disposable microfluidic electrochemical device. Talanta, v. 205, DEC 1 2019. Web of Science Citations: 2.
FAVA, ELSON LUIZ; SILVA, TIAGO ALMEIDA; DO PRADO, THIAGO MARTIMIANO; DE MORAES, FERNANDO CRUZ; FARIA, RONALDO CENSI; FATIBELLO-FILHO, ORLANDO. Electrochemical paper-based microfluidic device for high throughput multiplexed analysis. Talanta, v. 203, p. 280-286, OCT 1 2019. Web of Science Citations: 3.
PROENCA, CAMILA A.; BALDO, THAISA A.; FREITAS, TAYANE A.; MATERON, ELSA M.; WONG, ADEMAR; DURAN, ANDRES A.; MELENDEZ, MATIAS E.; ZAMBRANO, GUSTAVO; FARIA, RONALDO C. Novel enzyme-free immunomagnetic microfluidic device based on Co0.25Zn0.75Fe2O4 for cancer biomarker detection. Analytica Chimica Acta, v. 1071, p. 59-69, SEP 13 2019. Web of Science Citations: 3.
DE OLIVEIRA, TASSIA REGINA; FONSECA, WILSON TIAGO; SETTI, GRAZIELLE DE OLIVEIRA; FARIA, RONALDO CENSI. Fast and flexible strategy to produce electrochemical paper-based analytical devices using a craft cutter printer to create wax barrier and screen-printed electrodes. Talanta, v. 195, p. 480-489, APR 1 2019. Web of Science Citations: 7.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.