Development of simple electrochemical methods for the detection of biomarkers for the diagnosis of diseases

• Grant number: 17/24053-7
• Support Opportunities: Regular Research Grants
• Duration: June 01, 2018 - November 30, 2020
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Ronaldo Censi Faria
• Grantee: Ronaldo Censi Faria
• Host Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The development of simple, fast, and low-cost methods for protein biomarkers detection for disease diagnosis has been the focuses of our research group in the last years. For the next period, the aim is continuing the research in this area with focus on the development of new devices and methods to improve the scientific knowledge in the field and advances in technological innovation. In this new step, we intend to work in the microRNA detection for cancer diagnosis, that is a great challenge since we do not have previous experience with this type of analyte. This project will be carried out in partnership with Hospital do Câncer de Barretos, which we have a scientific and technical cooperation agreement and a project approved in ethical committees of both institutions. In the field of biomarkers detection, we have different works focusing on the protein detection for diagnosis of diseases as cancer and Alzheimer's. In this project, we will work in the development of low-cost and disposable electrochemical methods for applications with and without microfluidics. In this way, biosensors will be constructed by nanostructure film formed over the disposable electrodes following by covalent immobilization of antibodies for the protein biomarkers or DNA or RNA probes complementary to the targets microRNA. Magnetic particles havely decorated with antibodies or DNA or RNA probes will be used for capture, separation, preconcentration and detection of the analytes. The application of the analytical devices will be evaluated in patient and crop samples that will be provided by the partner research groups. The project has also the aim to form human resources in the different levels and the publication of the papers in national and international journals as well as the deposition of patents. (AU)

Scientific publications

(14)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FONSECA, WILSON T.; CINCOTTO, FERNANDO H.; LOURENCAO, BRUNA C.; DE ALMEIDA, V, STHEFANE; MORAES, FERNANDO C.; FATIBELLO-FILHO, ORLANDO; DE CARVALHO, ANA C.; CARVALHO, ANDRE L.; MELENDEZ, MATIAS E.; FARIA, RONALDO C.. Ultrasensitive magnetogenoassay for detection of microRNA for diagnosis of metastatic lymph nodes in head and neck cancer using disposable electrodes. SENSORS AND ACTUATORS B-CHEMICAL, v. 352, n. 1, FEB 1 2022. (16/16565-5, 17/24053-7, 16/12926-3, 20/01050-5)

FAVA, ELSON LUIZ; DO PRADO, THIAGO MARTIMIANO; SILVA, TIAGO ALMEIDA; DE MORAES, FERNANDO CRUZ; FARIA, RONALDO CENSI; FATIBELLO-FILHO, ORLANDO. New Disposable Electrochemical Paper-based Microfluidic Device with Multiplexed Electrodes for Biomarkers Determination in Urine Sample. Electroanalysis, v. 32, n. 5, JAN 2020. (17/24053-7, 16/10012-4, 17/10118-0)

FAVA, ELSON LUIZ; SILVA, TIAGO ALMEIDA; DO PRADO, THIAGO MARTIMIANO; DE MORAES, FERNANDO CRUZ; FARIA, RONALDO CENSI; FATIBELLO-FILHO, ORLANDO. Electrochemical paper-based microfluidic device for high throughput multiplexed analysis. Talanta, v. 203, p. 280-286, OCT 1 2019. (16/10012-4, 17/24053-7, 17/10118-0)

MATERON, ELSA MARIA; WONG, ADEMAR; FREITAS, TAYANE AGUIAR; FARIA, RONALDO CENSI; OLIVEIRA JR, OSVALDO N.. sensitive electrochemical detection of metronidazole in synthetic serum and urine samples using low-cost screen-printed electrodes modified with reduced graphene oxide and C6. JOURNAL OF PHARMACEUTICAL ANALYSIS, v. 11, n. 5, p. 646-652, OCT 2021. (17/24053-7, 18/22214-6)

MATERON, ELSA M.; NASCIMENTO, GUSTAVO F.; SHIMIZU, FLAVIO M.; CAMARA, AMANDA S.; SANDRINO, BIANCA; FARIA, RONALDO C.; OLIVEIRA JR, OSVALDO N.. y Role of sphingomyelin on the interaction of the anticancer drug gemcitabine hydrochloride with cell membrane models. COLLOIDS AND SURFACES B-BIOINTERFACES, v. 196, DEC 2020. (12/15543-7, 17/24053-7, 17/04712-6, 18/22214-6)

PROENCA, CAMILA A.; BALDO, THAISA A.; FREITAS, TAYANE A.; MATERON, ELSA M.; WONG, ADEMAR; DURAN, ANDRES A.; MELENDEZ, MATIAS E.; ZAMBRANO, GUSTAVO; FARIA, RONALDO C.. Novel enzyme-free immunomagnetic microfluidic device based on Co0.25Zn0.75Fe2O4 for cancer biomarker detection. Analytica Chimica Acta, v. 1071, p. 59-69, SEP 13 2019. (17/24053-7, 16/00991-5, 15/19890-1)

FAVA, ELSON LUIZ; DO PRADO, THIAGO MARTIMIANO; GARCIA-FILHO, AMAURI; SILVA, TIAGO ALMEIDA; CINCOTTO, FERNANDO HENRIQUE; DE MORAES, FERNANDO CRUZ; FARIA, RONALDO CENSI; FATIBELLO-FILHO, ORLANDO. Non-enzymatic electrochemical determination of creatinine using a novel screen-printed microcell. Talanta, v. 207, JAN 15 2020. (17/24053-7, 16/10012-4, 17/10118-0)

BALDO, THAISA APARECIDA; PROENCA, CAMILA DOS ANJOS; FELIX, FABIANA DA SILVA; FREITAS, TAYANE AGUIAR; SAKATA, SOLANGE KAZUMI; ANGNES, LUCIO; FARIA, RONALDO CENSI. Disposable electrochemical microfluidic device for ultrasensitive detection of egg allergen in wine samples. Talanta, v. 232, SEP 1 2021. (13/14262-7, 17/24053-7, 16/00991-5, 14/50867-3, 17/13137-5)

FONSECA, WILSON TIAGO; CASTRO, KARLA RIBEIRO; DE OLIVEIRA, TASSIA REGINA; FARIA, RONALDO CENSI. Disposable and Flexible Electrochemical Paper-based Analytical Devices Using Low-cost Conductive Ink. Electroanalysis, v. 33, n. 6, MAR 2021. (20/04635-4, 17/24053-7, 14/22401-0, 18/04332-1)

DE OLIVEIRA, TASSIA R.; ERBERELI, CAMILA R.; MANZINE, PATRICIA R.; MAGALHAES, THAMIRES N. C.; BALTHAZAR, MARCIO L. F.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Early Diagnosis of Alzheimer's Disease in Blood Using a Disposable Electrochemical Microfluidic Platform. ACS SENSORS, v. 5, n. 4, p. 1010-1019, APR 24 2020. (14/22401-0, 15/26084-1, 18/15571-7, 17/24839-0, 17/24053-7, 15/19890-1)

MATERON, ELSA M.; SHIMIZU, FLAVIO M.; DOS SANTOS, KEVIN FIGUEIREDO; NASCIMENTO, GUSTAVO F.; GERALDO, VANANELIA P. N.; OLIVEIRA JR, OSVALDO N.; FARIA, RONALDO C.. Membrane model as key tool in the study of glutathione-s-transferase mediated anticancer drug resistance. BIOMEDICINE & PHARMACOTHERAPY, v. 145, JAN 2022. (12/15543-7, 17/24053-7, 16/00991-5, 18/22214-6)

JONES, ABBY L.; DHANAPALA, LASANGI; BALDO, THAISA A.; SHARAFELDIN, MOHAMED; KRAUSE, COLLEEN E.; SHEN, MIN; MOGHADDAM, SHIRIN; FARIA, RONALDO C.; DEY, DIPAK K.; WATSON, R. WILLIAM; et al. Prostate Cancer Diagnosis in the Clinic Using an 8-Protein Biomarker Panel. Analytical Chemistry, v. 93, n. 2, p. 1059-1067, JAN 19 2021. (17/24053-7)

FREITAS, TAYANE A.; PROENCA, CAMILA A.; BALDO, THAISA A.; MATERON, ELSA M.; WONG, ADEMAR; MAGNANI, RODRIGO F.; FARIA, RONALDO C.. Ultrasensitive immunoassay for detection of Citrus tristeza virus in citrus sample using disposable microfluidic electrochemical device. Talanta, v. 205, DEC 1 2019. (17/24053-7, 16/00991-5, 15/19890-1)

DE OLIVEIRA, TASSIA REGINA; FONSECA, WILSON TIAGO; SETTI, GRAZIELLE DE OLIVEIRA; FARIA, RONALDO CENSI. Fast and flexible strategy to produce electrochemical paper-based analytical devices using a craft cutter printer to create wax barrier and screen-printed electrodes. Talanta, v. 195, p. 480-489, APR 1 2019. (17/24053-7, 14/22401-0, 15/19890-1)

Filed patent(s) as a result of this research project