Development of a CRISPR/Cas-based electrochemical biosensor for T-Cell Leukemia virus (HTLV-1) and Staphylococcus Aureus bacteria detection with point-of-care devices

• Grant number: 21/09706-0
• Support Opportunities: Scholarships in Brazil - Doctorate
• Effective date (Start): December 01, 2021
• Status: Discontinued
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Lauro Tatsuo Kubota
• Grantee: Dagwin Wachholz Júnior
• Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated research grant: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis, AP.TEM
• Associated scholarship(s): 24/07237-0 - Development of simple, low-cost, and field-deployable PAM-free CRISPR-based biosensors for multiplexed nucleic acid detection, BE.EP.DR

Abstract

Early and efficient diagnosis of diseases is essential for a correct choice of treatment, in order to avoid complications and increase the survival rate. The development of new technologies for detection of biomarkers appears as an alternative to the currently available methods, and one of the most interesting approaches today is the use of CRISPR/Cas systems for detection of nucleic acids. These systems, however, are based on fluorescence probes, presenting disadvantages when compared to electrochemical techniques. Thus, this project has the objective to use the innovative technology of CRISPR/Cas for the development of highly sensitive and selective point-of-care electrochemical biosensors for the simultaneous detection of genetic material from viruses and bacteria. The device will be developed with the immobilization of nucleotide sequences modified with electrochemical probes, which after contact with Cas enzymes and target genetic material, will be cleaved and the redox signal accompanied before and after insertion of the target sequence. This system will be developed to detect the virus that causes T-cell leukemia and the bacteria Staphylococcus aureus, but it will also allow the construction of a universal platform for detecting other diseases, by simply modifying the target sequences. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

WACHHOLZ JUNIOR, DAGWIN; DEROCO, PATRICIA BATISTA; KUBOTA, LAURO TATSUO. A copper-based metal-organic framework/reduced graphene oxide-modified electrode for electrochemical detection of paraquat. Microchimica Acta, v. 189, n. 8, p. 12-pg., 2022-08-01. (21/09706-0, 19/00166-2, 13/22127-2, 14/50867-3)

VIDOTTO, LOURENCO HENRIQUE BITTAR; WACHHOLZ, DAGWIN; KUBOTA, LAURO TATSUO. A simple and low-cost portable potentiostat with real-time data sharing for wireless electrochemical analyses. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 937, p. 9-pg., 2023-04-12. (14/50867-3, 21/09706-0, 13/22127-2)

DEROCO, PATRICIA BATISTA; WACHHOLZ JUNIOR, DAGWIN; KUBOTA, LAURO TATSUO. Paper-based Wearable Electrochemical Sensors: a New Generation of Analytical Devices. Electroanalysis, v. N/A, p. 12-pg., 2022-06-28. (21/14732-0, 14/50867-3, 13/22127-2, 19/00166-2, 21/09706-0)