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Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system

Abstract

This project proposes the development of analytical methods for the determination of protein biomarkers for the diagnosis of diseases as cancer and Alzheimer's. To achieve this goal a new full disposable microfluidic device to work with eight to twelve independent carbon electrode will be developed that will allow the multiplexed detection of protein biomarkers. This device will be constructed using low-cost equipment and materials, and it will be used simple microfluidic system using a syringe pump and a chromatographic valve for the sample injection. The analytical methods will be developed using magnetic particles heavily decorated with primary antibody and enzymatic labels for capture, separation, preconcentration of the analytes and signal amplification. With this, we expected to develop analytical methods for multiplexed determination of biomarkers with very low limit of detection, high selectivity and low-cost. The project has also the objective to produce papers in high-impact journals and primarily contribute to formation of high quality human resources for research. (AU)

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VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (10)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA, TASSIA R.; ERBERELI, CAMILA R.; MANZINE, PATRICIA R.; MAGALHAES, THAMIRES N. C.; BALTHAZAR, MARCIO L. F.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Early Diagnosis of Alzheimer's Disease in Blood Using a Disposable Electrochemical Microfluidic Platform. ACS SENSORS, v. 5, n. 4, p. 1010-1019, . (15/19890-1, 15/26084-1, 17/24053-7, 18/15571-7, 14/22401-0, 17/24839-0)
FREITAS, TAYANE A.; PROENCA, CAMILA A.; BALDO, THAISA A.; MATERON, ELSA M.; WONG, ADEMAR; MAGNANI, RODRIGO F.; FARIA, RONALDO C.. Ultrasensitive immunoassay for detection of Citrus tristeza virus in citrus sample using disposable microfluidic electrochemical device. Talanta, v. 205, . (17/24053-7, 16/00991-5, 15/19890-1)
MATERON, ELSA MARIA; WONG, ADEMAR; FATIBELLO-FILHO, ORLANDO; FARIA, RONALDO CENSI. Development of a simple electrochemical sensor for the simultaneous detection of anticancer drugs. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, v. 827, p. 64-72, . (15/19890-1)
ULIANA, CAROLINA V.; DE OLIVEIRA, TASSIA R.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Label-free evaluation of small-molecule-protein interaction using magnetic capture and electrochemical detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 10, p. 2111-2119, . (15/24940-8, 14/22401-0, 15/19890-1)
DE OLIVEIRA, RICARDO A. G.; MATERON, ELSA M.; MELENDEZ, MATIAS E.; CARVALHO, ANDRE L.; FARIA, RONALDO C.. Disposable Microfluidic Immunoarray Device for Sensitive Breast Cancer Biomarker Detection. ACS APPLIED MATERIALS & INTERFACES, v. 9, n. 33, p. 27433-27440, . (15/19890-1)
ULIANA, CAROLINA V.; PEVERARI, CAMILA R.; AFONSO, ANDRE S.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Fully disposable microfluidic electrochemical device for detection of estrogen receptor alpha breast cancer biomarker. BIOSENSORS & BIOELECTRONICS, v. 99, p. 156-162, . (15/19890-1, 13/06750-1, 12/24079-2)
DE OLIVEIRA, TASSIA REGINA; FONSECA, WILSON TIAGO; SETTI, GRAZIELLE DE OLIVEIRA; FARIA, RONALDO CENSI. Fast and flexible strategy to produce electrochemical paper-based analytical devices using a craft cutter printer to create wax barrier and screen-printed electrodes. Talanta, v. 195, p. 480-489, . (17/24053-7, 14/22401-0, 15/19890-1)
DE OLIVEIRA, TASSIA REGINA; MARTUCCI, DIEGO HENRIQUE; FARIA, RONALDO CENSI. Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay. SENSORS AND ACTUATORS B-CHEMICAL, v. 255, n. 1, p. 684-691, . (14/22401-0, 14/21115-3, 15/19890-1)
PROENCA, CAMILA A.; BALDO, THAISA A.; FREITAS, TAYANE A.; MATERON, ELSA M.; WONG, ADEMAR; DURAN, ANDRES A.; MELENDEZ, MATIAS E.; ZAMBRANO, GUSTAVO; FARIA, RONALDO C.. Novel enzyme-free immunomagnetic microfluidic device based on Co0.25Zn0.75Fe2O4 for cancer biomarker detection. Analytica Chimica Acta, v. 1071, p. 59-69, . (17/24053-7, 16/00991-5, 15/19890-1)
DE OLIVEIRA, RICARDO A. G.; CAMARGO, FIAMMA; PESQUERO, NAIRA C.; FARIA, RONALDO CENSI. A simple method to produce 2D and 3D microfluidic paper-based analytical devices for clinical analysis. Analytica Chimica Acta, v. 957, p. 40-46, . (15/19890-1)

Please report errors in scientific publications list by writing to: cdi@fapesp.br.
Filed patent(s) as a result of this research project

DISPOSITIVO PARA DETECÇÃO DO BIOMARCADOR ADAM10 PARA O DIAGNÓSTICO DA DOENÇA DE ALZHEIMER, MÉTODO DE APLICAÇÃO DO REFERIDO DISPOSITIVO, USO DO DITO DISPOSITIVO PARA DIAGNÓSTICO DA DOENÇA DE ALZHEIMER, MÉTODO DE APLICAÇÃO DE ELISA PARA DIAGNÓSTICO DA DOENÇA DE ALZHEIMER BR1020160181500 - Universidade Federal de São Carlos (UFSCAR) . Márcia Regina Cominetti; Ronaldo Censi Faria; Tássia Regina De Oliveira; Camila Regina Erbereli; Patricia Regina Manzine Moralles - August 2016, 04

VITROCERÂMICAS USINÁVEIS DE ALTA TENACIDADE À FRATURA E USO DAS MESMAS BR1020160181798 - Universidade Federal de São Carlos (UFSCAR) . Edgar Dutra Zanotto; Viviane Oliveira Soares - August 2016, 05

DISPOSITIVO PARA DETECÇÃO DO BIOMARCADOR ADAM10 PARA O DIAGNÓSTICO DA DOENÇA DE ALZHEIMER, MÉTODO DE APLICAÇÃO DO REFERIDO DISPOSITIVO, USO DO DITO DISPOSITIVO PARA DIAGNÓSTICO DA DOENÇA DE ALZHEIMER, MÉTODO DE APLICAÇÃO DE ELISA PARA DIAGNÓSTICO DA DOENÇA DE ALZHEIMER PCT/BR2017/000086 - Universidade Estadual de Campinas (UNICAMP) . Ronaldo Censi Faria; Tássia Regina de Oliveira; Camila Regina Erbereli; Patricia Regina Manzine; Moralles; Márcia Regina Cominetti - August 2017, 04

VITROCERÂMICAS USINÁVEIS DE ALTA TENACIDADE À FRATURA E USO DAS MESMAS PCT/BR2017/000087 - Universidade Estadual de Campinas (UNICAMP) . Edgar Dutra Zanotto; Viviane Oliveira Soares - August 2017, 04

DISPOSITIVO DESCARTÁVEL PARA DETECÇÃO DE BIOMARCADORES POR ELETROQUIMILUMINESCÊNCIA E MÉTODO DE OBTENÇÃO E DE DETECÇÃO BR1020180696009 - Universidade Federal de São Carlos (UFSCAR) . RONALDO CENSI FARIA ; TÁSSIA REGINA DE OLIVEIRA ; CAROLINA VENTURINI ULIANA - September 2018, 25

SENSOR ELETROQUÍMICO DESCARTÁVEL PARA QUANTIFICAÇÃO DE MIRNA PARA O DIAGNÓSTICO DE DOENÇAS E MÉTODO DE OBTENÇÃO E DE QUANTIFICAÇÃO BR1020200065211 - Fundação Pio XII ; Universidade Federal de São Carlos (UFSCAR) . RONADO CENSI FARIA ; WILSON TIAGO DA FONSECA ; FERNANDO HENRIQUE CINCOTTO ; ORLANDO FATIBELLO FILHO ; MATIAS ELISEO MELENDEZ ; ANA CAROLINA DE CARVALHO PETERS ; ANDRÉ LOPES CARVALHO - March 2020, 31