The objective of this project is to build two disposable microfluidic devices composed of a screen printed biosensor array (enzymatic and genosensors) containing 16 independent sensing points. From the prototype development, the multiplex determination of different species related to various abnormalities or diseases could be possible, with a low cost and using only one sample aliquot. The enzimatyc biosensors using the electrode array will be used for the multiplex determination of three different diseases in a unique urine sample. In this case, the arrangement will have a biosensor based on bilirubin oxidase for the determination of bilirubin using it as a sensor for the detection of hepatitis and cirrhosis, a biosensor based on creatinine amidohydrolase for creatinine detection (related to the emergence of kidney disease) and also a biosensor with glucose oxidase for the determination of glucose (for glucose monitoring in patients with development of glucosuria and / or diabetes). The second device to be developed will consist of a set of three genosensors to be applied for monitoring of genetic mutations linked to breast cancer. In this study three types of mutations in oligonucleotides will be monitored, the c.68_69del and c.52_66dup in BRCA1 and BRCA2 for c.59_46del. Thus, three genosensors will be constructed, each containing an oligonucleotide having a mutation, and the response is related to the hybridization of this oligonucleotide to its complementary strand. For genosensors with this arrangement, the possibility of monitoring the three genetic mutations in a single sample is expected, which in this case is of saliva and serum samples. This proposed study is a very innovative and promising analytical alternative, which will make possible not only the detection of target analytes, as well as carrying out rapid preventive diagnostics at a low cost.
News published in Agência FAPESP Newsletter about the scholarship: