Development of disposable immunosensors for detection of Alzheimer's Disease biomarkers

• Grant number: 14/22401-0
• Support type: Scholarships in Brazil - Doctorate
• Effective date (Start): March 01, 2015
• Effective date (End): February 28, 2019
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal researcher: Ronaldo Censi Faria
• Grantee: Tássia Regina de Oliveira
• Home Institution: Centro de Ciências Exatas e de Tecnologia (CCET). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil

Abstract

The Alzheimer Disease (AD) is a population aging disease with great economic and social impact in public health. In the project we will developed electrochemical immunosensors for the detection of ²A1-42, P-Tau, and ADAM10 protein biomarkers in plasma and serum samples of AD patients in different stages. The detection of AD biomarkers detections requires costly and time consuming techniques. In this sense, this project proposes the development of low-cost disposable electrochemical immunosensors for AD diagnosis. Disposable electrochemical cells will be constructed by screen-printed using carbon ink. The prepared electrodes will be modified with monoclonal antibodies by layer-by-layer technique and the detection will be carry out exposing the modified electrode to a solution containing the biomarkers. After the incubation time, the modified electrodes will be exposed to a solution containing the polyclonal antibody labeled with horseradish peroxidase (HRP). The detection step will be performed electrochemically by the enzymatic reaction of HRP using hydrogen peroxide as substrate and hydroquinone as redox mediator. The detection of analytes will be carry out in fetal bovine serum fortified with different biomarkers using the developed immunosensor. After this, the immunosensor will used in real samples of AD patients at several stages. Dr. Márcia Cominetti from Department of Gerontology of Federal University of São Carlos will collaborated in the project, she has a large experience in the AD research and will support us providing real samples of AD patients. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ULIANA, CAROLINA V.; DE OLIVEIRA, TASSIA R.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Label-free evaluation of small-molecule-protein interaction using magnetic capture and electrochemical detection. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 411, n. 10, p. 2111-2119, APR 2019. (15/24940-8, 14/22401-0, 15/19890-1)

DE OLIVEIRA, TASSIA R.; ERBERELI, CAMILA R.; MANZINE, PATRICIA R.; MAGALHAES, THAMIRES N. C.; BALTHAZAR, MARCIO L. F.; COMINETTI, MARCIA R.; FARIA, RONALDO C.. Early Diagnosis of Alzheimer's Disease in Blood Using a Disposable Electrochemical Microfluidic Platform. ACS SENSORS, v. 5, n. 4, p. 1010-1019, APR 24 2020. (15/19890-1, 18/15571-7, 17/24053-7, 15/26084-1, 14/22401-0, 17/24839-0)

FONSECA, WILSON TIAGO; CASTRO, KARLA RIBEIRO; DE OLIVEIRA, TASSIA REGINA; FARIA, RONALDO CENSI. Disposable and Flexible Electrochemical Paper-based Analytical Devices Using Low-cost Conductive Ink. Electroanalysis, v. 33, n. 6, MAR 2021. (14/22401-0, 18/04332-1, 17/24053-7, 20/04635-4)

DE OLIVEIRA, TASSIA REGINA; FONSECA, WILSON TIAGO; SETTI, GRAZIELLE DE OLIVEIRA; FARIA, RONALDO CENSI. Fast and flexible strategy to produce electrochemical paper-based analytical devices using a craft cutter printer to create wax barrier and screen-printed electrodes. Talanta, v. 195, p. 480-489, APR 1 2019. (14/22401-0, 15/19890-1, 17/24053-7)

DE OLIVEIRA, TASSIA REGINA; MARTUCCI, DIEGO HENRIQUE; FARIA, RONALDO CENSI. Simple disposable microfluidic device for Salmonella typhimurium detection by magneto-immunoassay. SENSORS AND ACTUATORS B-CHEMICAL, v. 255, n. 1, p. 684-691, FEB 2018. (14/22401-0, 15/19890-1, 14/21115-3)