Dendrimer-encapsulated nanoparticles as a sensing platform for Alzheimer´s biomarker detection

• Grant number: 18/14130-7
• Support type: Scholarships abroad - Research Internship - Post-doctor
• Effective date (Start): November 01, 2018
• Effective date (End): October 31, 2019
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Principal researcher: Lúcia Vieira
• Grantee: Claudia Do Amaral Razzino
• Supervisor abroad: Jose M. Pingarron
• Home Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Research place: Universidad Complutense de Madrid (UCM), Spain
• Associated to the scholarship: 15/26651-3 - Development of immunosensors for Alzheimer's biomarkers electrochemical detection, BP.PD

Abstract

The Alzheimer's disease (AD) is a chronic neurodegenerative disease. Early diagnosis is extremely necessary and desirable, as it allows access to therapies that alleviate the symptoms from the disease onset. The Tau protein is one of biomarkers for AD and may be detected in human serum to estimate the risk or presence of AD. Currently, methods for the detection of biomarker do not exhibit high sensitivity for diagnosis in early stages and costs are incompatible with the wide monitoring required. Thus, this project proposes the development and optimization of an electrochemical immunosensor (EI) based on the modification of screen printed electrodes with carbon nanotubes or reduced graphene oxide and dendrimer-encapsulated gold nanoparticles. Subsequently, the modified electrodes will be characterized by various techniques. The modification of surface electrode may significantly improve the electrochemical detection (ED) properties of EI developed by decreasing the electrode overpotential and increasing the current intensity and/or increased sensitivity. The antibodies are linked to nanostructures on the electrode surface through the formation of bonds using EDC/NHS. The biomarkers electrochemical detection occurs using an antibody labeled with the enzyme horseradish peroxidase (HRP). In the ED system, hydroquinone (HQ) is added to detection solution as an electronic mediator of the reaction between HRP and hydrogen peroxide (H2O2). In the presence of H2O2, HRP catalyzes the oxidation of HQ quinone, wherein the current reduction associated with this process is monitored. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SERAFIN, V; GAMELLA, M.; PEDRERO, M.; MONTERO-CALLE, A.; RAZZINO, C. A.; YANEZ-SEDENO, P.; BARDERAS, R.; CAMPUZANO, S.; PINGARRON, J. M.. Enlightening the advancements in electrochemical bioanalysis for the diagnosis of Alzheimer's disease and other neurodegenerative disorders. Journal of Pharmaceutical and Biomedical Analysis, v. 189, SEP 10 2020. (18/14130-7)

RAZZINO, CLAUDIA A.; SERAFIN, VERONICA; GAMELLA, MARIA; PEDRERO, MARIA; MONTERO-CALLE, ANA; BARDERAS, RODRIGO; CALERO, MIGUEL; LOBO, ANDERSON O.; YANEZ-SEDENO, PALOMA; CAMPUZANO, SUSANA; et al. An electrochemical immunosensor using gold nanoparticles-PAMAM-nanostructured screen-printed carbon electrodes for tau protein determination in plasma and brain tissues from Alzheimer patients. BIOSENSORS & BIOELECTRONICS, v. 163, SEP 1 2020. (18/14130-7)

SERAFIN, VERONICA; RAZZINO, CLAUDIA A.; GAMELLA, MARIA; PEDRERO, MARIA; POVEDANO, ELOY; MONTERO-CALLE, ANA; BARDERAS, RODRIGO; CALERO, MIGUEL; LOBO, ANDERSON O.; YANEZ-SEDENO, PALOMA; et al. Disposable immunoplatforms for the simultaneous determination of biomarkers for neurodegenerative disorders using poly(amidoamine) dendrimer/gold nanoparticle nanocomposite. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 413, n. 3, SI, MAY 2020. (18/14130-7)