Development of immunosensors for Alzheimer's biomarkers electrochemical detection

• Grant number: 15/26651-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Effective date (Start): March 01, 2017
• Effective date (End): February 28, 2021
• Field of knowledge: Engineering - Materials and Metallurgical Engineering - Nonmetallic Materials
• Acordo de Cooperação: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Lúcia Vieira
• Grantee: Claudia Do Amaral Razzino
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated research grant: 12/15857-1 - Scientific studies and innovation application on CVD diamond, DLC and carbon nanostructures obtained by chemical vapor deposition technique, AP.TEM
• Associated scholarship(s): 18/14130-7 - Dendrimer-encapsulated nanoparticles as a sensing platform for Alzheimer´s biomarker detection, BE.EP.PD

Abstract

The Alzheimer's disease (AD) is a chronic neurodegenerative disease. Early diagnosis is extremely necessary and desirable, as it allows access to therapies that alleviate the symptoms from the disease onset. The ²-amyloid and ApoE4 neurochemical biomarkers are indicators that may be detected in physiological fluids such as cerebrospinal fluid, serum and plasma, to estimate the risk or presence of AD. Currently, methods for the detection of these biomarkers in these fluids did not exhibit high sensitivity for diagnosis in early stages and costs are incompatible with the wide monitoring required. Thus, this project proposes the development and optimization of an electrochemical immunosensor (EI) based on the modification of conventional electrodes and screen printed electrodes with conductive polymer nanofibers (CPN). These CPN and the modified electrodes will be characterized by various techniques. With the electrodes modification is intended to improve significantly the electrochemical detection (ED) properties of EI developed by decreasing the electrode overpotential and increasing of current intensity and/or increased sensitivity. The antibodies are linked to nanostructures on the electrode surface through the formation of bonds using EDC/NHS. The biomarkers electrochemical detection occurs using an antibody labeled with the enzyme horseradish peroxidase (HRP). In the ED system, hydroquinone (HQ) is added to detection solution as an electronic mediator of the reaction between HRP and hydrogen peroxide (H2O2). In the presence of H2O2, HRP catalyzes the oxidation of HQ quinone, wherein the current reduction associated with this process is monitored. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GHOSH, ANUPAMA; RAZZINO, CLAUDIA DO AMARAL; DASGUPTA, ARCHI; FUJISAWA, KAZUNORI; VIEIRA, LAIS HELENA S.; SUBRAMANIAN, SHRUTI; COSTA, RUBENS S.; LOBO, ANDERSON O.; FERREIRA, ODAIR P.; ROBINSON, JOSHUA; et al. Structural and electrochemical properties of babassu coconut mesocarp-generated activated carbon and few-layer graphene. Carbon, v. 145, p. 175-186, APR 2019. (15/26651-3)