Transcranial low-level laser therapy in an in vivo model of stroke: Relevance to the brain infarct, microglia activation and neuroinflammation

Autor(es)

Vogel, Debora D. S. ^[1] ; Ortiz-Villatoro, Nancy N. ^[1] ; Araujo, Noemi S. ^[1] ; Marques, Marcia Jonathas Guimaraes ^[1] ; Aimbire, Flavio ^[2] ; Scorza, Fulvio A. ^[1] ; Scorza, Carla A. ^[1] ; Albertini, Regiane ^[3]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Escola Paulista Med EPM, Dept Neurol & Neurocirurg, Disciplina Neurociencia, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, Dept Ciencia & Tecnol, Programa Posgrad Med Translac, Sao Paulo - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, Dept Ciencia & Tecnol, Programa Posgrad Ciencia Movimento Humano & Reabi, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. 14, n. 6 MAR 2021.
• Citações Web of Science: 3

Resumo

Stroke is the main cause of death and functional disability. The available therapy affects only 5% of patients, and new therapeutic approaches have been constantly tested. Transcranial photobiomodulation (PBM) is promising for its neuroprotective effect on brain injuries. Thus, the present study investigated the PBM effects in an in vivo model of ischemic stroke induced by photothrombosis (PT). Five different groups of Wistar rats were submitted or not to a daily dose of fish oil or/and laser sessions for 2 months. The ischemia volume was evaluated by stereology; GFAP, Iba and NeuN by immunohistochemistry; TNF-alpha, IL-1 beta, IL-6, IL-10 and TGF-beta by ELISA assay. PBM influenced both the lesion volume and the GFAP. Furthermore, PBM and omega-3 or both reduced Iba RNAm. PBM reduced TNF-alpha, IL-1 beta, IL-6, brain damage, neuroinflammation and microglial activation, and it increased astroglial activity in peri-lesioned region after stroke. (AU)

• Processo FAPESP: 16/10286-7 - Avaliação do Papel da Suplementação com Ômega-3 e Laserterapia de Baixa Intensidade no Tálamo em Modelo Experimental de Acidente Vascular Cerebral Isquêmico
• Beneficiário: Regiane Albertini
• Modalidade de apoio: Auxílio à Pesquisa - Regular