Interleukin-8-251T > a, interleukin-1α-889C > t and apolipoprotein e polymorphisms in Alzheimer's disease

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Alex Augusto Vendramini ^[1] ; Roger Willian de Lábio ^[2] ; Lucas Trevizani Rasmussen ; Nathali Mattiuzo dos Reis ; Thais Minett ^[5] ; Paulo Henrique Ferreira Bertolucci ^[6] ; Marcela Augusta de Souza Pinhel ^[7] ; Dorotéia Rossi Silva Souza ^[8] ; Diego Robles Mazzotti ^[9] ; Marília de Arruda Cardoso Smith ^[10] ; Spencer Luiz Marques Payão

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^[1] Universidade do Sagrado Coração - Brasil

^[2] Faculdade de Medicina de Marília. Hemocentro - Brasil

^[5] Universidade Federal de São Paulo. Escola Paulista de Medicina - Brasil

^[6] Universidade Federal de São Paulo. Escola Paulista de Medicina - Brasil

^[7] Faculdade de Medicina de São José do Rio Preto - Brasil

^[8] Faculdade de Medicina de São José do Rio Preto - Brasil

^[9] Universidade Federal de São Paulo. Escola Paulista de Medicina - Brasil

^[10] Universidade Federal de São Paulo. Escola Paulista de Medicina - Brasil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 34, n. 1, p. 1-5, 2010-11-19.

Resumo

An inflammatory process has been involved in numerous neurodegenerative disorders such as Parkinson's disease, stroke and Alzheimer's disease (AD). In AD, the inflammatory response is mainly located in the vicinity of amyloid plaques. Cytokines, such as interleukin-8 (IL-8) and interleukin-1α (IL-1α), have been clearly involved in this inflammatory process. Polymorphisms of several interleukin genes have been correlated to the risk of developing AD. The present study investigated the association of AD with polymorphisms IL-8 -251T > A (rs4073) and IL-1α-889C > T (rs1800587) and the interactive effect of both, adjusted by the Apolipoprotein E genotype. 199 blood samples from patients with AD, 146 healthy elderly controls and 95 healthy young controls were obtained. DNA samples were isolated from blood cells, and the PCR-RFLP method was used for genotyping. The genotype distributions of polymorphisms IL-8, IL-1α and APOE were as expected under Hardy-Weinberg equilibrium. The allele frequencies did not differ significantly among the three groups tested. As expected, the APOE4 allele was strongly associated with AD (p < 0.001). No association of AD with either the IL-1α or the IL-8 polymorphism was observed, nor was any interactive effect between both polymorphisms. These results confirm previous studies in other populations, in which polymorphisms IL-8 -251T > A and IL-1α-889C > T were not found to be risk factors for AD. (AU)

• Processo FAPESP: 04/15273-3 - Caracterização dos genes ribossômicos e dos polimorfismos da apolipoproteína AV e das interleucinas 6, 8 e 1ª (alpha) em pacientes com Doença de Alzheimer
• Beneficiário: Spencer Luiz Marques Payão
• Modalidade de apoio: Auxílio à Pesquisa - Regular